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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Haemophilus ducreyi

NCTC Number: NCTC 10945
Current Name: Haemophilus ducreyi
Original Strain Reference: CIP 542
Other Collection No: ATCC 33940; CIP 54.2; CIP 542; DSM 8925; X2
Previous Catalogue Name: Haemophilus ducreyi
Type Strain: Yes
Family: Pasteurellaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: h.ducreyi medium,30, facultative anaerobe
Conditions for growth on liquid media: ,30, facultative anaerobe
16S rRNA Gene Sequence: >gb|Y11738|CIP 542|H.ducreyi DNA for 16S-23S rRNA spacer region, strain CIP 542.| ccaaaataaagacat... >gb|M63900 M38751|CIP 542|Haemophilus ducreyi 16S ribosomal RNA gene, 3 end.| gcaggcttaacacat... >gb|M63900 M38751|CIP 542|Haemophilus ducreyi 16S ribosomal RNA gene, 3' end.| gcaggcttaacacat...
23S rRNA Gene Sequence: >gb|Y11738|CIP 542|H.ducreyi DNA for 16S-23S rRNA spacer region, strain CIP 542.| ccaaaataaagacat... >gb|M55252 M38751|CIP 542|Haemophilus ducreyi 23S ribosomal RNA gene, partial.| agttaagtgactaag...
Extended Bibliography: showhide Show bibliography
Ref #: 95542
Author(s): Rossau,R.;Duhamel,M.;Jannes,G.;Decourt,J.L.;Van Heuverswyn,H.
Journal: J Gen Microbiol
Title: The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid
Volume: 137
Page(s): 277-85
Year: 1991
Keyword(s): GENBANK/M38751 GENBANK/M55252 GENBANK/M59337 GENBANK/M59338 GENBANK/M59339 GENBANK/M59340 GENBANK/M59341 GENBANK/M59342 GENBANK/M59343 GENBANK/M59344 GENBANK/M63900 Base Sequence Chancroid/microbiology Cloning, Molecular Haemophilus ducreyi/*classification/genetics Humans Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization *Oligonucleotide Probes Polymerase Chain Reaction RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Alignment
Remarks: Part of a ribosomal ribonucleic acid (rRNA) cistron of Haemophilus ducreyi was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 H. ducreyi strains and 13 or 14 non-H. ducreyi strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for H. ducreyi strains. Comparisons of 16S rRNA sequences confirm that H. ducreyi is a member of the Pasteurellaceae though not closely related to other species in this family.
URL: 1707945
Ref #: 95523
Author(s): Gu,X.X.;Rossau,R.;Jannes,G.;Ballard,R.;Laga,M.;Van Dyck,E.
Journal: Microbiology
Title: The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay
Volume: 144 ( Pt 4)
Page(s): 1013-9
Year: 1998
Keyword(s): GENBANK/Y11717 GENBANK/Y11738 Base Sequence Chancroid/diagnosis/microbiology DNA, Ribosomal/*isolation & purification Genes, Bacterial/*genetics Haemophilus ducreyi/genetics/*isolation & purification Humans Molecular Sequence Data Polymerase Chain Reaction/*methods Sequence Alignment Sequence Analysis, DNA
Remarks: The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.
URL: 9579075
Ref #: 12800
Author(s): Rossau,R.;Duhamel,M.;Jannes,G.;Decourt,J.L.;Van Heuverswyn,H.
Journal: J Gen Microbiol
Title: The development of specific rRNA-derived oligonucleotide probes for Haemophilus ducreyi, the causative agent of chancroid
Volume: 137 ( Pt 2)
Page(s): 277-85
Year: 1991
Keyword(s): GENBANK/M38751 GENBANK/M55252 GENBANK/M59337 GENBANK/M59338 GENBANK/M59339 GENBANK/M59340 GENBANK/M59341 GENBANK/M59342 GENBANK/M59343 GENBANK/M59344 GENBANK/M63900 Base Sequence Chancroid/microbiology Cloning, Molecular Haemophilus ducreyi/*classification/genetics Human Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization *Oligonucleotide Probes Polymerase Chain Reaction RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Alignment
Remarks: Part of a ribosomal ribonucleic acid (rRNA) cistron of Haemophilus ducreyi was enzymically amplified using conserved primers within the rRNA molecules, cloned in a plasmid vector, and sequenced. From the nucleotide sequence, eight oligonucleotides complementary to different regions in the 16S and 23S rRNA molecules were selected, chemically synthesized, and used as hybridization probes. Hybridization experiments with at least 41 H. ducreyi strains and 13 or 14 non-H. ducreyi strains revealed that all eight oligonucleotide probes were highly reliable and completely specific for H. ducreyi strains. Comparisons of 16S rRNA sequences confirm that H. ducreyi is a member of the Pasteurellaceae though not closely related to other species in this family.
URL: 91202097
Ref #: 13718
Author(s): Gu,X.X.;Rossau,R.;Jannes,G.;Ballard,R.;Laga,M.;Van Dyck,E.
Journal: Microbiology
Title: The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay
Volume: 144 ( Pt 4)
Page(s): 1013-9
Year: 1998
Keyword(s): GENBANK/Y11717 GENBANK/Y11738 Base Sequence Chancroid/diagnosis/microbiology DNA, Ribosomal/*isolation & purification Genes, Bacterial/*genetics Haemophilus ducreyi/genetics/*isolation & purification Human Molecular Sequence Data Polymerase Chain Reaction/*methods Sequence Alignment Sequence Analysis, DNA Support, Non-U.S. Gov't
Remarks: The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods. These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.
URL: 98240238
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 5066
Author(s): Carlone,G.M.;Schalla,W.O.;Moss,C.W.;Ashley,D.L.;Fast,D.M.;Holler,J.S.;Pjikaytis,B.D.
Journal: Int. J. Syst. Bacteriol.
Title: Haemophilus ducreyi isoprenoid quinone content and structure determination.
Volume: 38
Page(s): 249-253
Year: 1988
Ref #: 5067
Author(s): Casin,I.;Grimont,F.;Grimont,P.A.D.;Sanson-LePors,M.-J.
Journal: Int. J. Syst. Bacteriol.
Title: Lack of deoxyribonucleic acid relatedness between Haemophilus ducreyi and other Haemophilus species.
Volume: 35
Page(s): 23-25
Year: 1985
Ref #: 7016
Author(s): Busse,H.-J.;Bunka,S.;Hensel,A.;Lubitz,W.
Journal: Int. J. Syst. Bacteriol.
Title: Discrimination of members of the family Pasteurellaceae based on polyamine patterns.
Volume: 47
Page(s): 698-708
Year: 1997
Data: Type strain / M. Piechaud, Institut Pasteur, Paris in 1972 / P. Kirche Institut Pasteur, Hanoi, Vietnam in 1954
Accession Date: 01/01/1972
History: ISOLATED BY KIRSCHE INSTITUT PASTEUR VIETNAM IN 1954
Authority: (Neveu-Lemaire 1921) Bergey et al. 1923 (AL)
Depositor: PIECHAUD M
Taxonomy: TaxLink: S1389 (Haemophilus ducreyi (Neveu-Lemaire 1921) Bergey et al. 1923) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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