Bacteria Collection: Campylobacter sputorum biovar sputorum

NCTC Number:	NCTC 11367
Current Name:	Campylobacter sputorum biovar sputorum
Original Strain Reference:	Florent strain Wat
Other Collection No:	ATCC 33562; CCUG 11289; CIP 53.103; CIP 53103; DSM 5363; LMG 6447
Previous Catalogue Name:	Deposited as Campylobacter sputorum subsp. bubulus
Type Strain:	Yes
Family:	Campylobacteraceae
Hazard Group (ACDP):	2
Release Restrictions:	Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media:	Blood agar, 37°C, microaerophilic, 5 Days
Isolated From:	mammal, bull sperm
16S rRNA Gene Sequence:	Accession number DQ174151
23S rRNA Gene Sequence:	Accession number X67772
Bibliography:	On etal. (1998). Emended description of Campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans. Int J Syst Bacteriol 48 (1) 195-206.
Extended Bibliography:	Show bibliography Ref #: 95450 Author(s): Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P. Journal: Int J Syst Evol Microbiol Title: Genetic relatedness within the genus Campylobacter inferred from rpoB sequences Volume: 56 Page(s): 937-45 Year: 2006 Keyword(s): Bacterial Proteins/*genetics Base Sequence Campylobacter/*classification/*genetics DNA Primers DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics DNA-Directed RNA Polymerases/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Statistics Remarks: The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species. URL: 16627635 Ref #: 1300 Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed) Journal: Int. J. Syst. Bacteriol. Title: Approved Lists of Bacterial Names. Volume: 30 Page(s): 225-420 Year: 1980 Ref #: 4220 Author(s): Vandamme,P.;Falsen,E.;Rossau,R.;Hoste,B.;Segers,P.;Tytgat,R.;DeLey,J. Journal: Int. J. Syst. Bacteriol. Title: Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Volume: 41 Page(s): 88-103 Year: 1991 Ref #: 7079 Author(s): On,S.L.W.;Atabay,H.I.;Corry,J.E.L.;Harrington,C.S.;Vandamme,P. Journal: Int. J. Syst. Bacteriol. Title: Emended description of Campylobacter sputorum and revision of its infrasubspecific (biovar) divisions, including C. sputorum biovar paraureolyticus, a urease-producing variant from cattle and humans. Volume: 48 Page(s): 195-206 Year: 1998
Data:	(ATCC 33562, CCUG 11289, CIP 53103) Type strain / CIP in 1980 / A. Florent, Brussels / Bull sperm / Thouvenot, H. & Florent, A. (1854) Ann. Inst. Pasteur 86, 237
Accession Date:	01/01/1980
History:	ISOLATED BY FLORENT A, BRUSSELS
Authority:	On et al 1998
Depositor:	COLLECTION INSTITUT PASTEUR
Biosafety Responsibility:	It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.