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Bacteria Collection: Streptococcus oralis

NCTC Number: NCTC 11427
Current Name: Streptococcus oralis
Original Strain Reference: PB182, Carlsson LVG/1
Other Collection No: ATCC 35037; DSM 20627; KCTC 13048
Previous Catalogue Name: Streptococcus oralis
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, human mouth
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1123931
16S rRNA Gene Sequence: >gb|X58308 S70359|NCTC 11427 (T)|S.oralis 16S rRNA.| gaacgggtgagtnac... >gb|AF003932|ATCC 35037|Streptococcus oralis 16S ribosomal RNA gene, complete sequence.| tttttgatttgatcc... >gb|AY347551|ATCC 35037|Streptococcus oralis strain ATCC 35037 16S-23S ribosomal RNAintergenic spacer, complete sequence.| ctaaggataaggaac... >gb|AY485602|ATCC 35037|Streptococcus oralis strain ATCC 35037 16S small subunit ribosomalRNA gene, partial sequence.| gacgaacgctggcgg...
23S rRNA Gene Sequence: >gb|AY347551|ATCC 35037|Streptococcus oralis strain ATCC 35037 16S-23S ribosomal RNAintergenic spacer, complete sequence.| ctaaggataaggaac... >gb|AY485602|ATCC 35037|Streptococcus oralis strain ATCC 35037 16S small subunit ribosomalRNA gene, partial sequence.| gacgaacgctggcgg...
Miscellaneous Sequence Data: >gb|AJ617791|DSM 20627|Streptococcus oralis partial gyrB gene for DNA gyrase subunit B.| atcgaccgacggtgc...
Bibliography: BRIDGE P D & SNEATH P H A 1982 INT J SYST BACT 32 410 415; CARLSSON J 1965
Extended Bibliography: showhide Show bibliography
Ref #: 95509
Author(s): Majewski,J.;Zawadzki,P.;Pickerill,P.;Cohan,F.M.;Dowson,C.G.
Journal: J Bacteriol
Title: Barriers to genetic exchange between bacterial species: Streptococcus pneumoniae transformation
Volume: 182
Page(s): 1016-23
Year: 2000
Keyword(s): GENBANK/AF194507 GENBANK/AF194508 GENBANK/AF194509 GENBANK/AF194510 GENBANK/AF194511 GENBANK/AF194512 GENBANK/AF194513 GENBANK/AF194514 GENBANK/AF194515 GENBANK/AF194516 GENBANK/AF194517 GENBANK/AF194518 GENBANK/AF194519 GENBANK/AF194520 GENBANK/AF194521 GENBANK/AF194522 GENBANK/AF194523 GENBANK/AF194524 GENBANK/AF194525 GENBANK/AF194526 GENBANK/AF194527 GENBANK/AF194528 Base Pair Mismatch/*genetics DNA Repair/*genetics DNA, Bacterial/genetics DNA-Directed RNA Polymerases/genetics Molecular Sequence Data Phylogeny Recombination, Genetic Sequence Analysis, DNA Streptococcus/genetics Streptococcus pneumoniae/*genetics/growth & development *Transformation, Bacterial
Remarks: Interspecies genetic exchange is an important evolutionary mechanism in bacteria. It allows rapid acquisition of novel functions by transmission of adaptive genes between related species. However, the frequency of homologous recombination between bacterial species decreases sharply with the extent of DNA sequence divergence between the donor and the recipient. In Bacillus and Escherichia, this sexual isolation has been shown to be an exponential function of sequence divergence. Here we demonstrate that sexual isolation in transformation between Streptococcus pneumoniae recipient strains and donor DNA from related strains and species follows the described exponential relationship. We show that the Hex mismatch repair system poses a significant barrier to recombination over the entire range of sequence divergence (0.6 to 27%) investigated. Although mismatch repair becomes partially saturated, it is responsible for 34% of the observed sexual isolation. This is greater than the role of mismatch repair in Bacillus but less than that in Escherichia. The remaining non-Hex-mediated barrier to recombination can be provided by a variety of mechanisms. We discuss the possible additional mechanisms of sexual isolation, in view of earlier findings from Bacillus, Escherichia, and Streptococcus.
URL: 10648528
Ref #: 82396
Author(s): Bentley,R.W.;Leigh,J.A.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences
Volume: 41
Page(s): 487-94
Year: 1992
Keyword(s): GENBANK/S70322 GENBANK/S70323 GENBANK/S70324 GENBANK/X58301 GENBANK/X58302 GENBANK/X58303 GENBANK/X58304 GENBANK/X58305 GENBANK/X58306 GENBANK/X58307 GENBANK/X58308 GENBANK/X58309 GENBANK/X58310 GENBANK/X58311 GENBANK/X58312 GENBANK/X58313 GENBANK/X58314 GENBANK/X58315 GENBANK/X58316 GENBANK/X58317 GENBANK/X58318 GENBANK/X58319 GENBANK/X58320 GENBANK/X58321 GENBANK/X59028 GENBANK/X59029 GENBANK/X59030 GENBANK/X59031 GENBANK/X59032 GENBANK/X59061 etc. Base Sequence DNA, Bacterial Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Homology, Nucleic Acid Streptococcus/classification/*genetics
Remarks: The partial 16S rRNA sequences of 24 Streptococcus species were determined by reverse transcription. A comparative analysis of these sequences and the sequences of seven previously studied streptococcal species revealed the presence of several clusters within the genus. The clusters obtained from the sequence analysis agreed in general with the groups outlined on the basis of the results of nucleic acid hybridization studies, but there were some exceptions. The pyogenic group was extended to include Streptococcus agalactiae, S. parauberis, S. porcinus, and S. uberis. Four oral groups were discerned; these four groups centered on S. mutans, S. salivarius, S. anginosus, and S. oralis. Some species (e.g., S. suis and S. acidominimus) did not cluster with any particular group. Our findings are discussed in the context of data from other genetic and chemotaxonomic studies.
URL: 1720654
Ref #: 39176
Author(s): Roth,S.B.;Jalava,J.;Ruuskanen,O.;Ruohola,A.;Nikkari,S.
Journal: J Clin Microbiol
Title: Use of an oligonucleotide array for laboratory diagnosis of bacteria responsible for acute upper respiratory infections
Volume: 42
Page(s): 4268-74
Year: 2004
Keyword(s): Bacterial Infections/*diagnosis Base Sequence DNA Primers DNA Topoisomerases, Type I/genetics Humans Oligonucleotide Array Sequence Analysis/*methods Oligonucleotide Probes Polymerase Chain Reaction Respiratory Tract Infections/*diagnosis Sensitivity and Specificity
Remarks: We developed a diagnostic array of oligonucleotide probes targeting species-specific variable regions of the genes encoding topoisomerases GyrB and ParE of respiratory bacterial pathogens. Suitable broad-range primer sequences were designed based on alignment of gyrB/parE sequences from nine different bacterial species. These species included Corynebacterium diphtheriae, Fusobacterium necrophorum, Haemophilus influenzae, Legionella pneumophila, Moraxella catarrhalis, Mycoplasma pneumoniae, Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus pyogenes. Specific probe sequences were selected by comparative analysis against the European Bioinformatics Database, as well as gyrB/parE sequences generated for this study. To verify specificity, at least six initial oligonucleotide probe sequences per bacterial species were tested by hybridization on a solid glass support using culture collection strains as templates. Finally, three oligonucleotide probes per bacterial species were utilized to examine 65 middle ear fluid and 29 throat swab samples. The sensitivities of the developed assay compared to classic culture from middle ear fluid samples for H. influenzae, M. catarrhalis, and S. pneumoniae were 96 (93 for culture), 73 (93 for culture), and 100% (78% for culture), respectively. No cross-reactivity with bacterial species belonging to the normal oral flora was observed when the 29 throat swab samples were studied. The sensitivity of the assay to detect S. pyogenes from these samples was 93% (80% for culture). These results provide a proof of concept for the diagnostic use of microarray technology based on broad-range topoisomerase gene amplification, followed by hybridization and specific detection of bacterial species.
URL: 15365022
Ref #: 95462
Author(s): Ferrandiz,M.J.;Ardanuy,C.;Linares,J.;Garcia-Arenzana,J.M.;Cercenado,E.;Fleites,A.;de la Campa,A.G.
Journal: Antimicrob Agents Chemother
Title: New mutations and horizontal transfer of rpoB among rifampin-resistant Streptococcus pneumoniae from four Spanish hospitals
Volume: 49
Page(s): 2237-45
Year: 2005
Keyword(s): Amino Acid Sequence Anti-Bacterial Agents/*pharmacology Base Sequence DNA-Directed RNA Polymerases/chemistry/*genetics Drug Resistance, Bacterial/*genetics *Gene Transfer, Horizontal Hospitals Humans Microbial Sensitivity Tests Molecular Sequence Data *Mutation Phylogeny Pneumococcal Infections/epidemiology/microbiology Rifampin/*pharmacology Sequence Analysis, DNA Spain/epidemiology Streptococcus pneumoniae/*drug effects/genetics
Remarks: A total of 103 (0.7%) of 14,236 Streptococcus pneumoniae isolates collected in four Spanish hospitals from 1989 to 2003 were resistant to rifampin (MICs, 4 to 512 microg/ml). Only sixty-one (59.2%) of these isolates were available for molecular characterization. Resistance was mostly related to human immunodeficiency virus (HIV) infection in adult patients and to conjunctivitis in children. Thirty-six different pulsed-field gel electrophoresis patterns were identified among resistant isolates, five of which were related to international clones (Spain23F-1, Spain6B-2, Spain9V-3, Spain14-5, and clone C of serotype 19F), and accounted for 49.2% of resistant isolates. Single sense mutations at cluster N or I of the rpoB gene were found in 39 isolates, while double mutations, either at cluster I, at clusters I and II, or at clusters N and III, were found in 14 isolates. The involvement of the mutations in rifampin resistance was confirmed by genetic transformation. Single mutations at clusters N and I conferred MICs of 2 microg/ml and 4 to 32 microg/ml, respectively. Eight isolates showed high degrees of nucleotide sequence variations (2.3 to 10.8%) in rpoB, suggesting a recombinational origin for these isolates, for which viridans group streptococci are their potential gene donors. Although the majority of rifampin-resistant isolates were isolated from individual patients without temporal or geographical relationships, the clonal dissemination of rifampin-resistant isolates was observed among 12 HIV-infected patients in the two hospitals with higher rates of resistance.
URL: 15917517
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 13387
Author(s): Bentley,R.W.;Leigh,J.A.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences
Volume: 41
Page(s): 487-94
Year: 1992
Keyword(s): GENBANK/S70322 GENBANK/S70323 GENBANK/S70324 GENBANK/X58301 GENBANK/X58302 GENBANK/X58303 GENBANK/X58304 GENBANK/X58305 GENBANK/X58306 GENBANK/X58307 GENBANK/X58308 GENBANK/X58309 GENBANK/X58310 GENBANK/X58311 GENBANK/X58312 GENBANK/X58313 GENBANK/X58314 GENBANK/X58315 GENBANK/X58316 GENBANK/X58317 GENBANK/X58318 GENBANK/X58319 GENBANK/X58320 GENBANK/X58321 GENBANK/X59028 GENBANK/X59029 GENBANK/X59030 GENBANK/X59031 GENBANK/X59032 GENBANK/X59061 etc. Base Sequence DNA, Bacterial Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Homology, Nucleic Acid Streptococcus/classification/*genetics Support, Non-U.S. Gov't
Remarks: The partial 16S rRNA sequences of 24 Streptococcus species were determined by reverse transcription. A comparative analysis of these sequences and the sequences of seven previously studied streptococcal species revealed the presence of several clusters within the genus. The clusters obtained from the sequence analysis agreed in general with the groups outlined on the basis of the results of nucleic acid hybridization studies, but there were some exceptions. The pyogenic group was extended to include Streptococcus agalactiae, S. parauberis, S. porcinus, and S. uberis. Four oral groups were discerned; these four groups centered on S. mutans, S. salivarius, S. anginosus, and S. oralis. Some species (e.g., S. suis and S. acidominimus) did not cluster with any particular group. Our findings are discussed in the context of data from other genetic and chemotaxonomic studies.
URL: 92075550
Ref #: 2710
Author(s): Bridge,P.D.;Sneath,P.H.A.
Journal: Int. J. Syst. Bacteriol.
Title: Streptococcus gallinarum sp. nov. and Streptococcus oralis sp. nov.
Volume: 32
Page(s): 410-415
Year: 1982
Ref #: 3345
Author(s): Kilpper-Bälz,R.;Wenzig,P.;Schleifer,K.H.
Journal: Int. J. Syst. Bacteriol.
Title: Molecular relationships and classification of some viridans streptococci as Streptococcus oralis and emended description of Streptococcus oralis (Bridge and Sneath 1982).
Volume: 35
Page(s): 482-488
Year: 1985
Ref #: 3346
Author(s): Schmidhuber,S.;Ludwig,W.;Schleifer,K.H.
Journal: J. Clin. Microbiol.
Title: Construction of a DNA probe for the specific identification of Streptococcus oralis.
Volume: 26
Page(s): 1042-1044
Year: 1988
Data: (ATCC 35037) Type strain / P. H. A. Sneath, Leicester University in 1982 / J. Carlsson, Sweden in 1978 / Human mouth, 1965 / Carlsson, J. (1968) Odont. Revy. 19, 137-160 / Bridge, P. D. & Sneath, P. H. A. (1982) Int. J. syst. Bact. 32, 410-415 / Kilpper-Balz, R. et al. (1985) Int. J. syst. Bact. 35, 482
Accession Date: 01/01/1982
History: ISOLATED BY CARLSSON 1968 - SNEATH P H A LEICESTER UNIV. PRE:FR
Authority: Bridge and Sneath 1982
Depositor: SNEATH P H A
Taxonomy: TaxLink: S2854 (Streptococcus oralis Bridge and Sneath 1982) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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