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Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Peptostreptococcus anaerobius

NCTC Number: NCTC 11460
Current Name: Peptostreptococcus anaerobius
Original Strain Reference: A. Prevot 4372
Other Collection No: ATCC 27337; A PREVOT 4372; VPI 4330
Previous Catalogue Name: Peptostreptococcus anaerobius
Type Strain: Yes
Family: Peptostreptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 24-48 hours, 37°C, anaerobic
Conditions for growth on liquid media: viande-leure blood agar,brain heart infusion,37, strict anaerobe oxr:an
16S rRNA Gene Sequence: >gb|L04168|ATCC 27337|Peptostreptococcus anaerobius 16S ribosomal RNA gene, completesequence.| tttatgagagtttga... >gb|D14150|ATCC27337|GIFU 7882|Peptostreptococcus anaerobius gene for 16S ribosomal RNA.| agagtttgatcctgg... >gb|Z29059|ATCC 27337|P.anaerobius (clone LK51) 16S rRNA/23S rRNA intergenic DNA.| agcttgacatccctc... >gb|Z29060|ATCC 27337|P.anaerobius (clone LK54) 16S rRNA/23S rRNA intergenic DNA.| agcttgacatccctt... >gb|Z28984|ATCC 27337|P.anaerobius (clone LK16) 16S rRNA/23S rRNA intergenic DNA.| agcttgacatccctt...
23S rRNA Gene Sequence: >gb|Z29059|ATCC 27337|P.anaerobius (clone LK51) 16S rRNA/23S rRNA intergenic DNA.| agcttgacatccctc... >gb|Z29060|ATCC 27337|P.anaerobius (clone LK54) 16S rRNA/23S rRNA intergenic DNA.| agcttgacatccctt... >gb|Z28984|ATCC 27337|P.anaerobius (clone LK16) 16S rRNA/23S rRNA intergenic DNA.| agcttgacatccctt...
Bibliography: HOLDEMAN L V ET AL 1977 ANAEROBE LAN MAN VIRGINIA POLY 4TH ED 1 156
Extended Bibliography: showhide Show bibliography
Ref #: 67985
Author(s): Ezaki,T.;Li,N.;Hashimoto,Y.;Miura,H.;Yamamoto,H.
Journal: Int J Syst Bacteriol
Title: 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov
Volume: 44
Page(s): 130-6
Year: 1994
Keyword(s): GENBANK/D14136 GENBANK/D14137 GENBANK/D14138 GENBANK/D14139 GENBANK/D14140 GENBANK/D14141 GENBANK/D14142 GENBANK/D14143 GENBANK/D14144 GENBANK/D14145 GENBANK/D14146 GENBANK/D14147 GENBANK/D14148 GENBANK/D14149 GENBANK/D14150 GENBANK/D14151 GENBANK/D14152 GENBANK/D14153 GENBANK/D14154 GENBANK/D14155 Anaerobiosis Base Sequence DNA, Bacterial/*chemistry DNA, Ribosomal/*chemistry Molecular Sequence Data Peptococcaceae/*classification/*genetics RNA, Ribosomal, 16S/*genetics
Remarks: The 16S ribosomal DNA sequences of representative members of the family Peptococcaceae were determined. The members of the family examined were divided into the following four phylogenetic groups: Peptococcus niger ATCC 27731T (T = type strain), the Sarcina-Peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. Peptococcus niger, the type species of the family, was not related to other members of the family. Peptostreptococcus anaerobius ATCC 27337T, the type strain of the type species of the genus Peptostreptococcus, was closely related to Clostridium sordellii NCIB 10717T (level of sequence similarity, 85%). Sarcina ventriculi GIFU 7886, a spore-forming anaerobic gram-positive coccus, clustered with Clostridium perfringens ATCC 13124T at a similarity value of 91%. Members of the Sarcina-Peptostreptococcus anaerobius group clustered with clostridia at similarity values ranging from 85 to 91%. The type strains of Peptostreptococcus prevotii, Peptostreptococcus asaccharolyticus, Peptostreptococcus micros, and Peptostreptococcus magnus clustered at levels of sequence homology of 84 to 93%. This cluster was not included in the Peptococcus niger group or the Peptostreptococcus anaerobius group. Thus, these members of the genus Peptostreptococcus should be separated from the other members of the genus and also from members of the family Peptococcaceae. The sequence of Peptostreptococcus productus ATCC 27340T was different from the sequences of Peptostreptococcus anaerobius and Peptococcus niger. The sequence of Streptococcus hansenii ATCC 27752T, a strictly anaerobic strain, was different from the sequences of other streptococci; this strain clustered with Peptostreptococcus productus, coprococci, and ruminococci. Several phenotypic characteristics of Streptococcus hansenii ATCC 27752T were similar to characteristics of ruminococci.(ABSTRACT TRUNCATED AT 250 WORDS)
URL: 8123556
Ref #: 35697
Author(s): Paster,B.J.;Russell,J.B.;Yang,C.M.;Chow,J.M.;Woese,C.R.;Tanner,R.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the ammonia-producing ruminal bacteria Peptostreptococcus anaerobius, Clostridium sticklandii, and Clostridium aminophilum sp. nov
Volume: 43
Page(s): 107-10
Year: 1993
Keyword(s): GENBANK/L04166 GENBANK/L04167 GENBANK/L04168 GENBANK/M23927 GENBANK/M23929 GENBANK/M26494 GENBANK/M59083 GENBANK/M59084 GENBANK/M59090 GENBANK/M59095 GENBANK/M59107 Ammonia/*metabolism Animals Cattle Clostridium/*classification/isolation & purification/*metabolism Female Molecular Sequence Data Peptostreptococcus/*classification/isolation & purification/*metabolism Phylogeny Rumen/*microbiology
Remarks: In previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. Three isolates, strains C, FT (T = type strain), and SR, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. On the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. In this study, the 16S rRNA sequences of these isolates and related bacteria were determined to establish the phylogenetic positions of the organisms. The sequences of strains C, FT, and SR and reference strains of Peptostreptococcus anaerobius, Clostridium sticklandii, Clostridium coccoides, Clostridium aminovalericum, Acetomaculum ruminis, Clostridium leptum, Clostridium lituseburense, Clostridium acidiurici, and Clostridium barkeri were determined by using a modified Sanger dideoxy chain termination method. Strain C, a large coccus purported to belong to the genus Peptostreptococcus, was closely related to P. anaerobius, with a level of sequence similarity of 99.6%. Strain SR, a heat-resistant, short, rod-shaped organism, was closely related to C. sticklandii, with a level of sequence similarity of 99.9%. However, strain FT, a heat-resistant, pleomorphic, rod-shaped organism, was only distantly related to some clostridial species and P. anaerobius. On the basis of the sequence data, it was clear that strain FT warranted designation as a separate species. The closest known relative of strain FT was C. coccoides (level of similarity, only 90.6%). Additional strains that are phenotypically similar to strain FT were isolated in this study.(ABSTRACT TRUNCATED AT 250 WORDS)
URL: 8427801
Ref #: 12106
Author(s): Ezaki,T.;Li,N.;Hashimoto,Y.;Miura,H.;Yamamoto,H.
Journal: Int J Syst Bacteriol
Title: 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov
Volume: 44
Page(s): 130-6
Year: 1994
Keyword(s): GENBANK/D14136 GENBANK/D14137 GENBANK/D14138 GENBANK/D14139 GENBANK/D14140 GENBANK/D14141 GENBANK/D14142 GENBANK/D14143 GENBANK/D14144 GENBANK/D14145 GENBANK/D14146 GENBANK/D14147 GENBANK/D14148 GENBANK/D14149 GENBANK/D14150 GENBANK/D14151 GENBANK/D14152 GENBANK/D14153 GENBANK/D14154 GENBANK/D14155 Anaerobiosis Base Sequence DNA, Bacterial/*chemistry DNA, Ribosomal/*chemistry Molecular Sequence Data Peptococcaceae/*classification/*genetics RNA, Ribosomal, 16S/*genetics Support, Non-U.S. Gov't
Remarks: The 16S ribosomal DNA sequences of representative members of the family Peptococcaceae were determined. The members of the family examined were divided into the following four phylogenetic groups: Peptococcus niger ATCC 27731T (T = type strain), the Sarcina-Peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. Peptococcus niger, the type species of the family, was not related to other members of the family. Peptostreptococcus anaerobius ATCC 27337T, the type strain of the type species of the genus Peptostreptococcus, was closely related to Clostridium sordellii NCIB 10717T (level of sequence similarity, 85%). Sarcina ventriculi GIFU 7886, a spore-forming anaerobic gram-positive coccus, clustered with Clostridium perfringens ATCC 13124T at a similarity value of 91%. Members of the Sarcina-Peptostreptococcus anaerobius group clustered with clostridia at similarity values ranging from 85 to 91%. The type strains of Peptostreptococcus prevotii, Peptostreptococcus asaccharolyticus, Peptostreptococcus micros, and Peptostreptococcus magnus clustered at levels of sequence homology of 84 to 93%. This cluster was not included in the Peptococcus niger group or the Peptostreptococcus anaerobius group. Thus, these members of the genus Peptostreptococcus should be separated from the other members of the genus and also from members of the family Peptococcaceae. The sequence of Peptostreptococcus productus ATCC 27340T was different from the sequences of Peptostreptococcus anaerobius and Peptococcus niger. The sequence of Streptococcus hansenii ATCC 27752T, a strictly anaerobic strain, was different from the sequences of other streptococci; this strain clustered with Peptostreptococcus productus, coprococci, and ruminococci. Several phenotypic characteristics of Streptococcus hansenii ATCC 27752T were similar to characteristics of ruminococci.(ABSTRACT TRUNCATED AT 250 WORDS)
URL: 94169037
Ref #: 12435
Author(s): Paster,B.J.;Russell,J.B.;Yang,C.M.;Chow,J.M.;Woese,C.R.;Tanner,R.
Journal: Int J Syst Bacteriol
Title: Phylogeny of the ammonia-producing ruminal bacteria Peptostreptococcus anaerobius, Clostridium sticklandii, and Clostridium aminophilum sp. nov
Volume: 43
Page(s): 107-10
Year: 1993
Keyword(s): GENBANK/L04166 GENBANK/L04167 GENBANK/L04168 GENBANK/M23927 GENBANK/M23929 GENBANK/M26494 GENBANK/M59083 GENBANK/M59084 GENBANK/M59090 GENBANK/M59095 GENBANK/M59107 Ammonia/*metabolism Animal Cattle Clostridium/*classification/isolation & purification/*metabolism Comparative Study Female Molecular Sequence Data Peptostreptococcus/*classification/isolation & purification/*metabolism Phylogeny Rumen/*microbiology Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S.
Remarks: In previous studies, gram-positive bacteria which grew rapidly with peptides or an amino acid as the sole energy source were isolated from bovine rumina. Three isolates, strains C, FT (T = type strain), and SR, were considered to be ecologically important since they produced up to 20-fold more ammonia than other ammonia-producing ruminal bacteria. On the basis of phenotypic criteria, the taxonomic position of these new isolates was uncertain. In this study, the 16S rRNA sequences of these isolates and related bacteria were determined to establish the phylogenetic positions of the organisms. The sequences of strains C, FT, and SR and reference strains of Peptostreptococcus anaerobius, Clostridium sticklandii, Clostridium coccoides, Clostridium aminovalericum, Acetomaculum ruminis, Clostridium leptum, Clostridium lituseburense, Clostridium acidiurici, and Clostridium barkeri were determined by using a modified Sanger dideoxy chain termination method. Strain C, a large coccus purported to belong to the genus Peptostreptococcus, was closely related to P. anaerobius, with a level of sequence similarity of 99.6%. Strain SR, a heat-resistant, short, rod-shaped organism, was closely related to C. sticklandii, with a level of sequence similarity of 99.9%. However, strain FT, a heat-resistant, pleomorphic, rod-shaped organism, was only distantly related to some clostridial species and P. anaerobius. On the basis of the sequence data, it was clear that strain FT warranted designation as a separate species. The closest known relative of strain FT was C. coccoides (level of similarity, only 90.6%). Additional strains that are phenotypically similar to strain FT were isolated in this study.(ABSTRACT TRUNCATED AT 250 WORDS)
URL: 93152452
Data: (VPI 4330; ATCC 27337) Type strain / ATCC in 1982 / W. E. C. Moore, VPI / A. Prevot / Holdeman, L. V. et al. (1977) Anaerobe Laboratory Manual 4th ed. Virginia Polytechnic & State University, Blacksburg, Virginia, pp. 1-156
Accession Date: 01/01/1982
History: ISOLATED BY PREVOT A- MOORE W E C VPI- ATCC
Authority: (Natvig 1905) Kluyver and van Niel 1936 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S2277 (Peptostreptococcus anaerobius (Natvig 1905) Kluyver and van Niel 1936) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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