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Bacteria Collection: Campylobacter upsaliensis

NCTC Number: NCTC 11541
Current Name: Campylobacter upsaliensis
Original Strain Reference: C231
Other Collection No: ATCC 43954; C231; CCUG 14913; CNCTC 7748; CNW C231; DSM 5365; LMG 8850
Previous Catalogue Name: Campylobacter upsaliensis
Type Strain: Yes
Family: Campylobacteraceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Buffered Charcoal Yeast Extract agar (BCYE), 48 hours, 37°C, microaerophilic
Conditions for growth on liquid media: blood agar,37, microaerophilic
Isolated From: faeces;faeces fron dog with enteritis
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS1201848
16S rRNA Gene Sequence: >gb|AF497805|LMG 8850|Campylobacter upsaliensis strain LMG 8850 16S ribosomal RNA gene,partial sequence.| tggcggcgtgcctaa... >gb|DQ174157|CCUG 14913|Campylobacter upsaliensis strain CCUG 14913 16S ribosomal RNA (rrs)gene, partial sequence.| agtgaacgctggcgg... >gb|AY621113|ATCC 43954|Campylobacter upsaliensis strain ATCC 43954 16S ribosomal RNA gene,partial sequence.| gtgcctaatacatgc...
Bibliography: SANDSTEDT K ET AL 1983 CURR MICROBIOL 8 209
Extended Bibliography: showhide Show bibliography
Ref #: 31741
Author(s): Misawa,N.;Kawashima,K.;Kondo,F.;Kushima,E.;Kushima,K.;Vandamme,P.
Journal: Vet Microbiol
Title: Isolation and characterization of Campylobacter, Helicobacter, and Anaerobiospirillum strains from a puppy with bloody diarrhea
Volume: 87
Page(s): 353-64
Year: 2002
Keyword(s): GENBANK/AF497805 GENBANK/AF497806 GENBANK/AF497807 GENBANK/AF497808 GENBANK/AF497809 GENBANK/AF497810 GENBANK/AF497811 Animals Bacterial Proteins/metabolism Base Sequence DNA, Bacterial/chemistry/genetics Diarrhea/microbiology/*veterinary Dog Diseases/drug therapy/*microbiology Dogs Electrophoresis, Polyacrylamide Gel/veterinary Feces/microbiology Female Gram-Negative Bacteria/genetics/*isolation & purification/ultrastructure Gram-Positive Bacterial Infections/drug therapy/microbiology/*veterinary Microscopy, Electron/veterinary Molecular Sequence Data Polymerase Chain Reaction/veterinary RNA, Ribosomal, 16S/chemistry/genetics
Remarks: We carried out a microscopic examination of stools from a 2-month-old female puppy with bloody diarrhea, and this revealed large numbers of different spiral-shaped bacteria. To isolate these organisms, a rectal swab specimen was inoculated onto plates of Skirrow's agar and incubated at 37 degrees C for 6 days in a microaerobic atmosphere. Finally, a total of six different spiral-shaped bacteria (strains G1104, 94105, FR106, B0101, 3J102, and J2103) were isolated. Based on their morphology, biochemical traits, whole-cell protein profiles, and analysis of their 16S rDNA sequences, they were identified as Campylobacter upsaliensis, Helicobacter cinaedi, 'Flexispira rappini', two Anaerobiospirillum spp. with different morphologies, and Helicobacter sp., respectively. Analysis of 16S rRNA gene sequence data for strains 94150 (H. cinaedi) and FR106 (F. rappini) revealed that this approach has limitations when identifying isolates to the species level because of a high degree of sequence homology between these species (>99%) and considerable sequence variation among different isolates within these species. The dog was treated orally with amoxicillin for 3 days, which resolved the diarrhea. However, 1 day after the last dose the bloody diarrhea recurred but regarded to six more days amoxicillin treatment. This suggests a bacterial cause for the diarrhea. The approach to identification to microaerobic spiral-shaped bacteria in diarrheic dogs can be applied further to characterize their role in diarrhea illness.
URL: 12069772
Ref #: 95450
Author(s): Korczak,B.M.;Stieber,R.;Emler,S.;Burnens,A.P.;Frey,J.;Kuhnert,P.
Journal: Int J Syst Evol Microbiol
Title: Genetic relatedness within the genus Campylobacter inferred from rpoB sequences
Volume: 56
Page(s): 937-45
Year: 2006
Keyword(s): Bacterial Proteins/*genetics Base Sequence Campylobacter/*classification/*genetics DNA Primers DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics DNA-Directed RNA Polymerases/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Statistics
Remarks: The genus Campylobacter comprises 17 species, some of which are important animal and human pathogens. To gain more insight into the genetic relatedness of this genus and to improve the molecular tools available for diagnosis, a universal sequencing approach was established for the gene encoding the beta-subunit of RNA polymerase (rpoB) for the genus Campylobacter. A total of 59 strains, including the type strains of currently recognized species as well as field isolates, were investigated in the study. A primer set specific for Campylobacter species enabled straightforward amplification and sequencing of a 530 bp fragment of the rpoB gene. The 16S rRNA gene sequences of all of the strains were determined in parallel. A good congruence was obtained between 16S rRNA and rpoB gene sequence-based trees within the genus Campylobacter. The branching of the rpoB tree was similar to that of the 16S rRNA gene tree, even though a few discrepancies were observed for certain species. The resolution of the rpoB gene within the genus Campylobacter was generally much higher than that of the 16S rRNA gene sequence, resulting in a clear separation of most species and even some subspecies. The universally applicable amplification and sequencing approach for partial rpoB gene sequence determination provides a powerful tool for DNA sequence-based discrimination of Campylobacter species.
URL: 16627635
Ref #: 12371
Author(s): Misawa,N.;Kawashima,K.;Kondo,F.;Kushima,E.;Kushima,K.;Vandamme,P.
Journal: Vet Microbiol
Title: Isolation and characterization of Campylobacter, Helicobacter, and Anaerobiospirillum strains from a puppy with bloody diarrhea
Volume: 87
Page(s): 353-64
Year: 2002
Keyword(s): GENBANK/AF497805 GENBANK/AF497806 GENBANK/AF497807 GENBANK/AF497808 GENBANK/AF497809 GENBANK/AF497810 GENBANK/AF497811 Animal Bacterial Proteins/metabolism Base Sequence Case Report DNA, Bacterial/chemistry/genetics Diarrhea/microbiology/*veterinary Dog Diseases/drug therapy/*microbiology Dogs Electrophoresis, Polyacrylamide Gel/veterinary Feces/microbiology Female Gram-Negative Bacteria/genetics/*isolation & purification/ultrastructure Gram-Positive Bacterial Infections/drug therapy/microbiology/*veterinary Microscopy, Electron/veterinary Molecular Sequence Data Polymerase Chain Reaction/veterinary RNA, Ribosomal, 16S/chemistry/genetics
Remarks: We carried out a microscopic examination of stools from a 2-month-old female puppy with bloody diarrhea, and this revealed large numbers of different spiral-shaped bacteria. To isolate these organisms, a rectal swab specimen was inoculated onto plates of Skirrow's agar and incubated at 37 degrees C for 6 days in a microaerobic atmosphere. Finally, a total of six different spiral-shaped bacteria (strains G1104, 94105, FR106, B0101, 3J102, and J2103) were isolated. Based on their morphology, biochemical traits, whole-cell protein profiles, and analysis of their 16S rDNA sequences, they were identified as Campylobacter upsaliensis, Helicobacter cinaedi, 'Flexispira rappini', two Anaerobiospirillum spp. with different morphologies, and Helicobacter sp., respectively. Analysis of 16S rRNA gene sequence data for strains 94150 (H. cinaedi) and FR106 (F. rappini) revealed that this approach has limitations when identifying isolates to the species level because of a high degree of sequence homology between these species (>99%) and considerable sequence variation among different isolates within these species. The dog was treated orally with amoxicillin for 3 days, which resolved the diarrhea. However, 1 day after the last dose the bloody diarrhea recurred but regarded to six more days amoxicillin treatment. This suggests a bacterial cause for the diarrhea. The approach to identification to microaerobic spiral-shaped bacteria in diarrheic dogs can be applied further to characterize their role in diarrhea illness.
URL: 22066012
Ref #: 3940
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 37.
Volume: 41
Page(s): 331
Year: 1991
Ref #: 4220
Author(s): Vandamme,P.;Falsen,E.;Rossau,R.;Hoste,B.;Segers,P.;Tytgat,R.;DeLey,J.
Journal: Int. J. Syst. Bacteriol.
Title: Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov.
Volume: 41
Page(s): 88-103
Year: 1991
Ref #: 4332
Author(s): Moss,C.W.;Lambert-Fair,M.A.;Nicholson,M.A.;Guerrant,G.
Journal: J. Clin. Microbiol.
Title: Isoprenoid quinones of Campylobacter cryaerophila, C. cinaedi, C. fennelliae, C. hyointestinalis, C. pylori, and "C. upsaliensis".
Volume: 28
Page(s): 395-397
Year: 1990
Ref #: 4739
Author(s): Goossens,H.;Pot,B.;Vlaes,L.;vandenBorre,C.;vandenAbbeele,R.;vanNaelten,C.;Levy,J.;Cogniau,H.;Marbehant,P.;Verhoef,J.;Kersters
Journal: J. Clin. Microbiol.
Title: Characterization and description of "Campylobacter upsaliensis" isolated from human feces.
Volume: 28
Page(s): 1039-1046
Year: 1990
Ref #: 5020
Author(s): Sandstedt,K.;Ursing,J.
Journal: System. Appl. Microbiol.
Title: Description of Campylobacter upsaliensis sp. nov. previously known as the CNW group.
Volume: 14
Page(s): 39-45
Year: 1991
Data: (ATCC 43954) Type strain / J. Ursing, Malmo, Sweden in 1983 / Faeces, dog / Sandstedt, K. et al. (1983) Curr. Microbiol. 8, 209 / Sandstedt, K. et al. (1991) Int. J. syst. Bact. 41, 331
Accession Date: 01/01/1983
History: ISOLTED BY SANDSTEDT K NAT VET INST UPPSALA - URSING J
Authority: Sandstedt and Ursing 1991
Depositor: URSING J
Taxonomy: TaxLink: S4108 (Campylobacter upsaliensis Sandstedt and Ursing 1991) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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