Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Bacteroides stercoris

NCTC Number: NCTC 13053
Current Name: Bacteroides stercoris
Other Collection No: ATCC 43183; JCM 9496
Type Strain: Yes
Family: Bacteroidaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Fastidious anaerobe agar, 24-48 hours, 37°C, anaerobic
23S rRNA Gene Sequence: >gb|AY155593|ATCC 43183|Bacteroides stercoris 23S ribosomal RNA gene, partial sequence.| aagcagccattcatt...
Extended Bibliography: showhide Show bibliography
Ref #: 25269
Author(s): Liu,C.;Song,Y.;McTeague,M.;Vu,A.W.;Wexler,H.;Finegold,S.M.
Journal: FEMS Microbiol Lett
Title: Rapid identification of the species of the Bacteroides fragilis group by multiplex PCR assays using group- and species-specific primers
Volume: 222
Page(s): 9-16
Year: 2003
Keyword(s): Bacteriological Techniques Bacteroides fragilis/*classification/*genetics/growth & development DNA Transposable Elements/genetics Phenotype Polymerase Chain Reaction/*methods RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics
Remarks: We report a rapid and reliable two-step multiplex polymerase chain reaction (PCR) assay to identify the 10 Bacteroides fragilis group species - Bacteroides caccae, B. distasonis, B. eggerthii, B. fragilis, B. merdae, B. ovatus, B. stercoris, B. thetaiotaomicron, B. uniformis and B. vulgatus. These 10 species were first divided into three subgroups by multiplex PCR-G, followed by three multiplex PCR assays with three species-specific primer mixtures for identification to the species level. The primers were designed from nucleotide sequences of the 16S rRNA, the 16S-23S rRNA intergenic spacer region and part of the 23S rRNA gene. The established two-step multiplex PCR identification scheme was applied to the identification of 155 clinical isolates of the B. fragilis group that were previously identified to the species level by phenotypic tests. The new scheme was more accurate than phenotypic identification, which was accurate only 84.5% of the time. The multiplex PCR scheme established in this study is a simple, rapid and reliable method for the identification of the B. fragilis group species. This will permit more accurate assessment of the role of various B. fragilis group members in infections and of the degree of antimicrobial resistance in each of the group members.
URL: 12757940
Accession Date: 14/06/2007
Taxonomy: TaxLink: S499 (Bacteroides stercoris johnson et al. 1986) - Date of change: 31/05/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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Ampoule (Bacteria)

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