Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Pandoraea sputorum

NCTC Number: NCTC 13161
Current Name: Pandoraea sputorum
Other Collection No: LMG 18819
Type Strain: Yes
Family: Burkholderiaceae
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24-48 hours, 37°C, aerobic
16S rRNA Gene Sequence: >gb|AF139176|LMG 18819|Pandoraea sputorum 16S ribosomal RNA gene, partial sequence.| attgaacgctggcgg...
Extended Bibliography: showhide Show bibliography
Ref #: 31256
Author(s): Coenye,T.;Falsen,E.;Hoste,B.;Ohlen,M.;Goris,J.;Govan,J.R.;Gillis,M.;Vandamme,P.
Journal: Int J Syst Evol Microbiol
Title: Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov
Volume: 50 Pt 2
Page(s): 887-99
Year: 2000
Keyword(s): GENBANK/AF139171 GENBANK/AF139172 GENBANK/AF139173 GENBANK/AF139174 GENBANK/AF139175 GENBANK/AF139176 Base Composition Betaproteobacteria/chemistry/*classification/genetics/physiology Burkholderia/classification/genetics Burkholderia Infections/microbiology Cystic Fibrosis/*microbiology DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Electrophoresis, Polyacrylamide Gel Fatty Acids/analysis Genes, rRNA Humans Molecular Sequence Data Nucleic Acid Hybridization Phenotype Phylogeny Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA *Soil Microbiology Sputum/*microbiology
Remarks: A polyphasic taxonomic study was performed on a group of isolates tentatively identified as Burkholderia cepacia, Ralstonia pickettii or Ralstonia paucula (formerly known as CDC group IVc-2). The isolates were mainly cultured from sputum of cystic fibrosis patients or from soil. SDS-PAGE of whole-cell proteins and AFLP fingerprinting distinguished at least five different species, and this was confirmed by DNA-DNA hybridizations. 16S rDNA sequence analysis of representative strains indicated that these organisms belong to the beta-subclass of the Proteobacteria, with the genera Burkholderia and Ralstonia as closest neighbours. Based on genotypic and phenotypic characteristics, the organisms were classified in a novel genus, Pandoraea. The DNA base composition of the members of the new genus is between 61.2 and 64.3 mol%. This novel genus includes four new species, Pandoraea apista (the type species) (type strain is LMG 16407T), Pandoraea pulmonicola (type strain is LMG 18106T), Pandoraea pnomenusa (type strain is LMG 18087T) and Pandoraea sputorum (type strain is LMG 18819T), and Pandoraea norimbergensis (Wittke et al. 1997) comb. nov. (type strain is LMG 18379T). The available clinical data indicate that at least some of these organisms may cause chronic infection in, and can be transmitted amongst, cystic fibrosis patients.
URL: 10758901
Accession Date: 14/06/2007
Taxonomy: TaxLink: ? (Pandoraea sputorum) - Date of change 14/06/2007
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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