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Escherichia coli

Escherichia coli

Catalogue No.

NCTC 14377

Current Name

Escherichia coli

Type Strain

Reference Strain

Original Strain Reference

MC1000 + pDM1-mcr-1

Culture Conditions

Growth Conditions on Solid Media

Nutrient agar, 37°C, O₂, 24 hours

Biological and Genomic Info

Strain Data

This organism has been genetically modified and is GMO2 / pDM1 is a tetracycline selectable synthetic plasmid containing a p15a ori that is maintained at ~10 copies per cell. mcr-1 is cloned into the multiple cloning site of this plasmid, and is IPTG-inducible and driven by a Ptac promoter.pDM1 sequence: Genbank MN128719.The sequence of the mcr-1 ORF is available on request.

Additional Info

Accession Date

17/09/2019

Depositor

Despoina Mavridou (Imperial College London, Department of Life Sciences, Kensington, London, UK)

Isolated From

GMO organism derived from Escherichia coli MC1000. London, UK.

History

Accessioned into the NCTC < Engineered by Despoina Mavridou

Applications

Bibliography

M J Casadaban, S N Cohen. J Mol Biol. 1980 Apr;138(2):179-207.L Dortet et al. Rapid detection and discrimination of chromosome- and MCR-plasmid-mediated resistance to polymyxins by MALDI-TOF MS in Escherichia coli: the MALDIxin test. J Antimicrob Chemother. 2018 Dec 1;73(12):3359-3367.

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Escherichia coli (NCTC 14377)

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

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