Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Streptococcus equi subsp. zooepidemicus

NCTC Number: NCTC 7023
Current Name: Streptococcus equi subsp. zooepidemicus
Original Strain Reference: S 34
Other Collection No: ATCC 43079; CCUG 23256; CIP 103228; DSM 20727; LMG 16030; NCIMB 701359
Previous Catalogue Name: Streptococcus equi subsp. Zooepidemicus
Type Strain: No
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group c
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: not recorded
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS879590
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/d...
16S rRNA Gene Sequence: >gb|AB002516|ATCC 43079|Streptococcus equi DNA for 16S rRNA, strain ATCC 43079.| cagattgatacgagc... >gb|EF406034|ATCC 43079|Streptococcus equi subsp. zooepidemicus strain ATCC 43079 16Sribosomal RNA gene, partial sequence.| gacgaacgctggcgg...
23S rRNA Gene Sequence: >gb|AB168121|ATCC 43079|Streptococcus equi subsp. zooepidemicus gene for 23S rRNA, completesequence, strain:ATCC 43079.| ggttaagttaataag... >gb|AJ549077|TYPE STRAIN: DSM 20727T|Streptococcus equi subsp. zooepidemicus 23S rRNA gene, type strainDSM 20727T.| ttcattcaaagagtg...
Extended Bibliography: showhide Show bibliography
Ref #: 95479
Author(s): Mitterer,G.;Huber,M.;Leidinger,E.;Kirisits,C.;Lubitz,W.;Mueller,M.W.;Schmidt,W.M.
Journal: J Clin Microbiol
Title: Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences
Volume: 42
Page(s): 1048-57
Year: 2004
Keyword(s): Abortion, Veterinary/microbiology Animals Bacteria/*genetics/*isolation & purification Base Sequence DNA Primers DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/*genetics/isolation & purification Female Horses *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Pregnancy Pregnancy Complications, Infectious/microbiology/veterinary RNA, Bacterial/genetics/isolation & purification RNA, Ribosomal, 23S/*genetics/isolation & purification
Remarks: The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. We describe a DNA microarray-based PCR approach for the quick detection and identification of bacteria from cervical swab specimens from mares. This on-chip PCR method combines the amplification of a variable region of bacterial 23S ribosomal DNA and the simultaneous sequence-specific detection on a solid phase. The solid phase contains bacterial species-specific primers covalently bound to a glass support. During the solid-phase amplification reaction the polymerase elongates perfectly matched primers and incorporates biotin-labeled nucleotides. The reaction products are visualized by streptavidin-cyanine 5 staining, followed by fluorescence scanning. This procedure successfully identified from pure cultures 22 bacteria that are common causes of abortion and sterility in mares. Using the on-chip PCR method, we also tested 21 cervical swab specimens from mares for the presence of pathogenic bacteria and compared the results with those of conventional bacteriological culture methods. Our method correctly identified the bacteria in 12 cervical swab samples, 8 of which contained more than one bacterial species. Due to the higher sensitivity of the on-chip PCR, this method identified bacteria in five cervical swab samples which were not detected by the conventional identification procedure. Our results show that this method will have great potential to be incorporated into the routine microbiology laboratory.
URL: 15004052
Ref #: 17178
Author(s): Kawata,K.;Anzai,T.;Senna,K.;Kikuchi,N.;Ezawa,A.;Takahashi,T.
Journal: FEMS Microbiol Lett
Title: Simple and rapid PCR method for identification of streptococcal species relevant to animal infections based on 23S rDNA sequence
Volume: 237
Page(s): 57-64
Year: 2004
Keyword(s): GENBANK/AB168118 GENBANK/AB168119 GENBANK/AB168120 GENBANK/AB168121 GENBANK/AB168122 GENBANK/AB168123 GENBANK/AB168124 GENBANK/AB168125 GENBANK/AB168126 GENBANK/AB168127 GENBANK/AB168128 Animals DNA, Bacterial/*analysis/chemistry DNA, Ribosomal/*analysis/chemistry Electrophoresis, Agar Gel Genes, rRNA Molecular Sequence Data Phylogeny Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Streptococcal Infections/microbiology/*veterinary Streptococcus/*classification/genetics/*isolation & purification
Remarks: A PCR identification system targeting 23S rDNA sequences for the identification of eight streptococcal species relevant to animal infections (Streptococcus agalactiae, S. bovis, S. canis, S. dysgalactiae, S. equi, S. porcinus, S. suis and S. uberis) was developed. This system consists of two PCR reactions, A and B, in which seven and eight primers, respectively, are used simultaneously, and was designed so that each amplification product indicates a species by its size. A total of 111 cultures, including the type strain of eight species, could be successfully identified and differentiated as individual species, except for the cross reactivity between S. bovis and S. equinus. The developed PCR system can complete the identification procedure for eight streptococcal species through two tube reactions per isolate, and, therefore, might provide a rapid, simple and accurate diagnostic tool for veterinary laboratories.
URL: 15268938
Ref #: 2833
Journal: Int. J. Syst. Bacteriol.
Title: Validation of the publication of new names and new combinations previously effectively published outside the IJSB. List No. 17.
Volume: 35
Page(s): 223-225
Year: 1985
Ref #: 3217
Author(s): Farrow,J.A.E.;Collins,M.D.
Journal: System. Appl. Microbiol.
Title: Taxonomic studies on streptococci of serological groups C, G and L and possibly related taxa.
Volume: 5
Page(s): 483-493
Year: 1984
Data: Dr Paterson, Edgware in 1945 / A. Sorsby / A. W. Stableforth, Weybridge / Sulphonamide resistant / Patent strain, unrestricted
Accession Date: 01/01/1945
History: RECEIVED FROM SORSBY A;STABLEFORTH A W ;REDHILL HOSPITAL PRE:FR
Authority: Farrow and Collins 1985
Depositor: PATERSON
Taxonomy: TaxLink: S2837 (Streptococcus equi subspecies zooepidemicus (ex frost and englebrecht 1936) farrow and collins 1985) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 7023) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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