Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Mycobacterium tuberculosis

NCTC Number: NCTC 7416
Current Name: Mycobacterium tuberculosis
Original Strain Reference: H 37 Rv
Other Collection No: ATCC 9360; H 37 RV
Previous Catalogue Name: Mycobacterium tuberculosis
Type Strain: No
Family: Mycobacteriaceae
Hazard Group (ACDP): 3
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: lowenstein-jensen,37, aerobic
Conditions for growth on liquid media: lowenstein-jensen,dorset egg,kirchner medium,37, aerobic oxr:ae
Isolated From: human
16S rRNA Gene Sequence: >gb|X58890 S45301|NCTC 7416 H37RV|M.tuberculosis ribosomal RNA operon encoding 16S and 23S ribosomalRNA.| ctgcagcgatgctat...
23S rRNA Gene Sequence: >gb|X58890 S45301|NCTC 7416 H37RV|M.tuberculosis ribosomal RNA operon encoding 16S and 23S ribosomalRNA.| ctgcagcgatgctat...
Bibliography: KUBICA G P ET AL 1972 INT J SYST BACT 22 99;ALLEN B W 1969 J MED LAB
Extended Bibliography: showhide Show bibliography
Ref #: 61085
Author(s): Kempsell,K.E.;Ji,Y.E.;Estrada,I.C.;Colston,M.J.;Cox,R.A.
Journal: J Gen Microbiol
Title: The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA
Volume: 138 Pt 8
Page(s): 1717-27
Year: 1992
Keyword(s): GENBANK/X58889 GENBANK/X58890 GENBANK/X58891 Base Sequence DNA, Bacterial Exons Molecular Sequence Data Mycobacterium leprae/*genetics Mycobacterium tuberculosis/*genetics Nucleic Acid Conformation *Operon *Promoter Regions (Genetics) RNA Precursors/*genetics RNA Processing, Post-Transcriptional RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Restriction Mapping Transcription, Genetic
Remarks: Mycobacterium tuberculosis H37Rv has a single rrn (ribosomal RNA) operon. The operon was cloned and a region of 1536 nucleotides was sequenced, starting 621 bp upstream from the 5'-end of the 16S rRNA coding region and continuing to the start of the 23S rRNA coding region. The 16S rRNA sequence inferred from the gene sequence was found to differ in one position from Mycobacterium bovis (nucleotide 1443) and from Mycobacterium microti (nucleotide 427). A single putative promoter was identified on the basis of similarities with the sequence of rrn operons of Bacillus subtilis and Escherichia coli. The regions of similarity include a -35 box, a -10 box, a stringent response element, antitermination signals, potential RNAase III processing sites and features of precursor rRNA secondary structure. Sequences upstream from the 5'-end of Mycobacterium leprae 16S rRNA were also investigated. Homologous schemes of secondary structure were deduced for precursor rRNA of both M. tuberculosis and M. leprae; although the principal features are common to both species there are notable differences.
URL: 1382114
Ref #: 13189
Author(s): Kempsell,K.E.;Ji,Y.E.;Estrada,I.C.;Colston,M.J.;Cox,R.A.
Journal: J Gen Microbiol
Title: The nucleotide sequence of the promoter, 16S rRNA and spacer region of the ribosomal RNA operon of Mycobacterium tuberculosis and comparison with Mycobacterium leprae precursor rRNA
Volume: 138 ( Pt 8)
Page(s): 1717-27
Year: 1992
Keyword(s): GENBANK/X58889 GENBANK/X58890 GENBANK/X58891 Base Sequence Comparative Study DNA, Bacterial Exons Molecular Sequence Data Mycobacterium leprae/*genetics Mycobacterium tuberculosis/*genetics Nucleic Acid Conformation *Operon *Promoter Regions (Genetics) RNA Precursors/*genetics RNA Processing, Post-Transcriptional RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Restriction Mapping Support, Non-U.S. Gov't Transcription, Genetic
Remarks: Mycobacterium tuberculosis H37Rv has a single rrn (ribosomal RNA) operon. The operon was cloned and a region of 1536 nucleotides was sequenced, starting 621 bp upstream from the 5'-end of the 16S rRNA coding region and continuing to the start of the 23S rRNA coding region. The 16S rRNA sequence inferred from the gene sequence was found to differ in one position from Mycobacterium bovis (nucleotide 1443) and from Mycobacterium microti (nucleotide 427). A single putative promoter was identified on the basis of similarities with the sequence of rrn operons of Bacillus subtilis and Escherichia coli. The regions of similarity include a -35 box, a -10 box, a stringent response element, antitermination signals, potential RNAase III processing sites and features of precursor rRNA secondary structure. Sequences upstream from the 5'-end of Mycobacterium leprae 16S rRNA were also investigated. Homologous schemes of secondary structure were deduced for precursor rRNA of both M. tuberculosis and M. leprae; although the principal features are common to both species there are notable differences.
URL: 92407516
Data: (ATCC 9360) ATCC in 1948 / Virulent variant / Streptomycin assay / Allen, B. W. (1969) J. med. lab. Technol. 26, 389 / Kubica, G. P. et al. (1972) Int. J. syst. Bact. 22, 99
Accession Date: 01/01/1948
Authority: (Zopf 1883) Lehmann and Neumann 1896 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S1987 (Mycobacterium tuberculosis (Zopf 1883) Lehmann and Neumann 1896) - Date of change: 5/02/2003
Other: H 37 RV, Human typeANTIBIOTIC (AND OTHER ANTIMICROBIALS) ASSAY, SENSITIVITY, RESISTANCE CONTROL STRAINSStreptomycin
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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Ampoule (Bacteria)
Bacterial DNA - 2µg

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