Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Streptococcus pneumoniae

NCTC Number: NCTC 7465
Current Name: Streptococcus pneumoniae
Original Strain Reference: SV 1
Other Collection No: ATCC 33400; DSM 20566
Previous Catalogue Name: Streptococcus pneumoniae
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar serotype 1
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic, requires carbon dioxide
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: not recorded
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/SAMEA2479568
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AJ001247|NCTC 7465 T|Streptococcus pneumoniae 16S ribosomal RNA, 3 end, strain NCTC7465 T.| cccggggtttagtgc... >gb|AJ001246|NCTC 7465 T|Streptococcus pneumoniae 16S ribosomal RNA, 5 end, strain NCTC7465 T.| gctggcggcgtgcct... >gb|X58312|NCTC 7465(T)|S.pneumoniae 16S rRNA.| gaacgggtgagtaac... >gb|AF003930|ATCC 33400|Streptococcus pneumoniae 16S ribosomal RNA gene, complete sequence.| atttgatcctggctc... >gb|AY347557|ATCC 33400|Streptococcus pneumoniae strain ATCC 33400 16S-23S ribosomal RNAintergenic spacer, complete sequence.| ctaaggataaggaac... >gb|AY485600|ATCC 33400|Streptococcus pneumoniae strain ATCC 33400 16S small subunitribosomal RNA gene, partial sequence.| gacgaacgctggcgg... >gb|AJ001247|NCTC 7465 T|Streptococcus pneumoniae 16S ribosomal RNA, 3' end, strain NCTC7465 T.| cccggggtttagtgc... >gb|AJ001246|NCTC 7465 T|Streptococcus pneumoniae 16S ribosomal RNA, 5' end, strain NCTC7465 T.| gctggcggcgtgcct...
23S rRNA Gene Sequence: >gb|AY347557|ATCC 33400|Streptococcus pneumoniae strain ATCC 33400 16S-23S ribosomal RNAintergenic spacer, complete sequence.| ctaaggataaggaac... >gb|AY485600|ATCC 33400|Streptococcus pneumoniae strain ATCC 33400 16S small subunitribosomal RNA gene, partial sequence.| gacgaacgctggcgg... >gb|AB168128|ATCC 33400|Streptococcus pneumoniae gene for 23S rRNA, complete sequence,strain:ATCC 33400.| ggttaagttaataag...
Extended Bibliography: showhide Show bibliography
Ref #: 82396
Author(s): Bentley,R.W.;Leigh,J.A.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences
Volume: 41
Page(s): 487-94
Year: 1992
Keyword(s): GENBANK/S70322 GENBANK/S70323 GENBANK/S70324 GENBANK/X58301 GENBANK/X58302 GENBANK/X58303 GENBANK/X58304 GENBANK/X58305 GENBANK/X58306 GENBANK/X58307 GENBANK/X58308 GENBANK/X58309 GENBANK/X58310 GENBANK/X58311 GENBANK/X58312 GENBANK/X58313 GENBANK/X58314 GENBANK/X58315 GENBANK/X58316 GENBANK/X58317 GENBANK/X58318 GENBANK/X58319 GENBANK/X58320 GENBANK/X58321 GENBANK/X59028 GENBANK/X59029 GENBANK/X59030 GENBANK/X59031 GENBANK/X59032 GENBANK/X59061 etc. Base Sequence DNA, Bacterial Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Homology, Nucleic Acid Streptococcus/classification/*genetics
Remarks: The partial 16S rRNA sequences of 24 Streptococcus species were determined by reverse transcription. A comparative analysis of these sequences and the sequences of seven previously studied streptococcal species revealed the presence of several clusters within the genus. The clusters obtained from the sequence analysis agreed in general with the groups outlined on the basis of the results of nucleic acid hybridization studies, but there were some exceptions. The pyogenic group was extended to include Streptococcus agalactiae, S. parauberis, S. porcinus, and S. uberis. Four oral groups were discerned; these four groups centered on S. mutans, S. salivarius, S. anginosus, and S. oralis. Some species (e.g., S. suis and S. acidominimus) did not cluster with any particular group. Our findings are discussed in the context of data from other genetic and chemotaxonomic studies.
URL: 1720654
Ref #: 17178
Author(s): Kawata,K.;Anzai,T.;Senna,K.;Kikuchi,N.;Ezawa,A.;Takahashi,T.
Journal: FEMS Microbiol Lett
Title: Simple and rapid PCR method for identification of streptococcal species relevant to animal infections based on 23S rDNA sequence
Volume: 237
Page(s): 57-64
Year: 2004
Keyword(s): GENBANK/AB168118 GENBANK/AB168119 GENBANK/AB168120 GENBANK/AB168121 GENBANK/AB168122 GENBANK/AB168123 GENBANK/AB168124 GENBANK/AB168125 GENBANK/AB168126 GENBANK/AB168127 GENBANK/AB168128 Animals DNA, Bacterial/*analysis/chemistry DNA, Ribosomal/*analysis/chemistry Electrophoresis, Agar Gel Genes, rRNA Molecular Sequence Data Phylogeny Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Streptococcal Infections/microbiology/*veterinary Streptococcus/*classification/genetics/*isolation & purification
Remarks: A PCR identification system targeting 23S rDNA sequences for the identification of eight streptococcal species relevant to animal infections (Streptococcus agalactiae, S. bovis, S. canis, S. dysgalactiae, S. equi, S. porcinus, S. suis and S. uberis) was developed. This system consists of two PCR reactions, A and B, in which seven and eight primers, respectively, are used simultaneously, and was designed so that each amplification product indicates a species by its size. A total of 111 cultures, including the type strain of eight species, could be successfully identified and differentiated as individual species, except for the cross reactivity between S. bovis and S. equinus. The developed PCR system can complete the identification procedure for eight streptococcal species through two tube reactions per isolate, and, therefore, might provide a rapid, simple and accurate diagnostic tool for veterinary laboratories.
URL: 15268938
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 13387
Author(s): Bentley,R.W.;Leigh,J.A.;Collins,M.D.
Journal: Int J Syst Bacteriol
Title: Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences
Volume: 41
Page(s): 487-94
Year: 1992
Keyword(s): GENBANK/S70322 GENBANK/S70323 GENBANK/S70324 GENBANK/X58301 GENBANK/X58302 GENBANK/X58303 GENBANK/X58304 GENBANK/X58305 GENBANK/X58306 GENBANK/X58307 GENBANK/X58308 GENBANK/X58309 GENBANK/X58310 GENBANK/X58311 GENBANK/X58312 GENBANK/X58313 GENBANK/X58314 GENBANK/X58315 GENBANK/X58316 GENBANK/X58317 GENBANK/X58318 GENBANK/X58319 GENBANK/X58320 GENBANK/X58321 GENBANK/X59028 GENBANK/X59029 GENBANK/X59030 GENBANK/X59031 GENBANK/X59032 GENBANK/X59061 etc. Base Sequence DNA, Bacterial Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/*genetics Sequence Homology, Nucleic Acid Streptococcus/classification/*genetics Support, Non-U.S. Gov't
Remarks: The partial 16S rRNA sequences of 24 Streptococcus species were determined by reverse transcription. A comparative analysis of these sequences and the sequences of seven previously studied streptococcal species revealed the presence of several clusters within the genus. The clusters obtained from the sequence analysis agreed in general with the groups outlined on the basis of the results of nucleic acid hybridization studies, but there were some exceptions. The pyogenic group was extended to include Streptococcus agalactiae, S. parauberis, S. porcinus, and S. uberis. Four oral groups were discerned; these four groups centered on S. mutans, S. salivarius, S. anginosus, and S. oralis. Some species (e.g., S. suis and S. acidominimus) did not cluster with any particular group. Our findings are discussed in the context of data from other genetic and chemotaxonomic studies.
URL: 92075550
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 3345
Author(s): Kilpper-Bälz,R.;Wenzig,P.;Schleifer,K.H.
Journal: Int. J. Syst. Bacteriol.
Title: Molecular relationships and classification of some viridans streptococci as Streptococcus oralis and emended description of Streptococcus oralis (Bridge and Sneath 1982).
Volume: 35
Page(s): 482-488
Year: 1985
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: (DSM 20566) Type strain / S.D. Elliott in 1948 / Rockefeller Institute for Medical Research / Type 1 / Patent strain, unrestricted
Accession Date: 01/01/1948
History: FROM THE ROCKEFELLER INST. USA,1948
Authority: (Klein 1884) Chester 1901 (AL)
Depositor: ELLIOTT S D
Taxonomy: TaxLink: S2858 (Streptococcus pneumoniae (Klein 1884) Chester 1901) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

Additional Information

Note: Links open in a new window

Note:

The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

Cultures supplied by Culture Collections are to be used as controls for microbiology testing and for research purposes only. Please view the Terms & Conditions of Supply for more information.

Contact us if you want to discuss commercial use of the cultures.

Available Formats

Ampoule (Bacteria)

Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.