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Bacteria and Mycoplasmas detail

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Bacteria Collection: Enterococcus durans

NCTC Number: NCTC 8307
Current Name: Enterococcus durans
Original Strain Reference: 98D Sherman
Other Collection No: ATCC 19432; CCM 5612; DSM 20633; JCM 8725; NCDO 596; 98D SHERMAN
Previous Catalogue Name: Enterococcus durans
Type Strain: Yes
Family: Enterococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar group d
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
16S rRNA Gene Sequence: >gb|AJ276354|DSM20633|Enterococcus durans 16S rRNA gene, strain DSM20633.| tcctggctcaggacg... >gb|X87177|ATCC 19432|E.durans 16S-23S rRNA spacer DNA & tRNA-Ala gene.| ctaaggaatattacg... >gb|X87178|ATCC 19432|E.durans 16S-23S rRNA spacer DNA.| ctaaggaatattacg... >gb|X87177|ATCC 19432|E.durans 16S-23S rRNA spacer DNA & tRNA-Ala gene.| ctaaggaatattacg...
23S rRNA Gene Sequence: >gb|AJ295304|[WL_] ATCC 19432 T|Enterococcus durans 23S rRNA gene, strain [WL_] ATCC 19432 T.| ggttaagtgaataag... >gb|AF273484|ATCC19432|Enterococcus durans 23S ribosomal RNA gene, partial sequence.| gtagtttgatattga... >gb|X87177|ATCC 19432|E.durans 16S-23S rRNA spacer DNA & tRNA-Ala gene.| ctaaggaatattacg... >gb|X87178|ATCC 19432|E.durans 16S-23S rRNA spacer DNA.| ctaaggaatattacg... >gb|X87177|ATCC 19432|E.durans 16S-23S rRNA spacer DNA & tRNA-Ala gene.| ctaaggaatattacg...
Bibliography: SHERMAN J M & WING H U 1937 J DAIRY SCI 20 165; SHATTOCK P M F 1949 J GEN
Extended Bibliography: showhide Show bibliography
Ref #: 43856
Author(s): Naimi,A.;Beck,G.;Branlant,C.
Journal: Microbiology
Title: Primary and secondary structures of rRNA spacer regions in enterococci
Volume: 143 ( Pt 3)
Page(s): 823-34
Year: 1997
Keyword(s): GENBANK/X87177 GENBANK/X87178 GENBANK/X87179 GENBANK/X87180 GENBANK/X87181 GENBANK/X87182 GENBANK/X87183 GENBANK/X87184 GENBANK/X87185 GENBANK/X87186 GENBANK/X87187 GENBANK/X87188 GENBANK/X87189 GENBANK/X87190 GENBANK/X87191 Base Sequence Enterococcus/*genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial/chemistry/*genetics RNA, Ribosomal/chemistry/*genetics RNA, Transfer/genetics Sequence Alignment
Remarks: The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods.
URL: 9084166
Ref #: 43380
Author(s): Tsiodras,S.;Gold,H.S.;Coakley,E.P.;Wennersten,C.;Moellering RC,J.r.;Eliopoulos,G.M.
Journal: J Clin Microbiol
Title: Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species
Volume: 38
Page(s): 3991-3
Year: 2000
Keyword(s): Animals Enterococcus/*genetics/isolation & purification Genes, Bacterial Genes, rRNA/*genetics Gram-Positive Bacterial Infections/*microbiology Humans Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 23S/chemistry/*genetics Sequence Analysis, DNA *Variation (Genetics)
Remarks: The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species of Enterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, and Enterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed from E. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species.
URL: 11060057
Ref #: 13719
Author(s): Naimi,A.;Beck,G.;Branlant,C.
Journal: Microbiology
Title: Primary and secondary structures of rRNA spacer regions in enterococci
Volume: 143 ( Pt 3)
Page(s): 823-34
Year: 1997
Keyword(s): GENBANK/X87177 GENBANK/X87178 GENBANK/X87179 GENBANK/X87180 GENBANK/X87181 GENBANK/X87182 GENBANK/X87183 GENBANK/X87184 GENBANK/X87185 GENBANK/X87186 GENBANK/X87187 GENBANK/X87188 GENBANK/X87189 GENBANK/X87190 GENBANK/X87191 Base Sequence Enterococcus/*genetics Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial/chemistry/*genetics RNA, Ribosomal/chemistry/*genetics RNA, Transfer/genetics Sequence Alignment Support, Non-U.S. Gov't
Remarks: The 16S-23S and 23S-5S rRNA spacer DNA regions (spacer regions 1 and 2, respectively) from Enterococcus faecalis, Enterococcus faecium, Enterococcus hirae, Enterococcus durans and Enterococcus mundtii were amplified by PCR. Their nucleotide sequences were established and a secondary structure model showing the interaction between the two spacer regions was built. Whereas lactococci and Streptococcus sensu stricto are characterized by a single type of spacer region 1, the enterococci show a high degree of variability in this region; thus the spacer regions 1 with and without tRNA(Ala) were characterized. However, as shown for lactococci and Streptococcus sensu stricto, the tRNA(Ala) gene does not encode the 3'-terminal CCA trinucleotide. A putative antitermination signal is found downstream from the tRNA(Ala) gene. Based on comparison with Lactococcus lactis and Streptococcus thermophilus, a double-stranded processing stem is proposed. In E, hirae, one of the three different types of spacer region 1 contains no tRNA(Ala), but displays a 107 nt insertion that forms a long stem-loop structure. A similar insertion (115 nt in length) was found in E. faecium and base compensatory mutations preserve the ability to form the long stem-loop structure. Such insertions may correspond to mobile intervening sequences, as found in the 23S rRNA coding sequences of some Gram-negative bacteria. The spacer regions 1 and 2 from the three subgroups of streptococci were compared, and except for the tRNA(Ala) gene and the double-stranded processing sites, little similarity was found, which opens large possibilities for future development of DNA-based typing methods.
URL: 97237711
Ref #: 13705
Author(s): Tsiodras,S.;Gold,H.S.;Coakley,E.P.;Wennersten,C.;Moellering RC,J.r.;Eliopoulos,G.M.
Journal: J Clin Microbiol
Title: Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species
Volume: 38
Page(s): 3991-3
Year: 2000
Keyword(s): Animal Enterococcus/*genetics/isolation & purification Genes, Bacterial Genes, rRNA/*genetics Gram-Positive Bacterial Infections/*microbiology Human Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 23S/chemistry/*genetics Sequence Analysis, DNA *Variation (Genetics)
Remarks: The highly conserved central loop of domain V of 23S RNA (nucleotides 2042 to 2628; Escherichia coli numbering) is implicated in peptidyltransferase activity and represents one of the target sites for macrolide, lincosamide, and streptogramin B antibiotics. DNA encoding domain V (590 bp) of several species of Enterococcus was amplified by PCR. Twenty enterococcal isolates were tested, including Enterococcus faecium (six isolates), Enterococcus faecalis, Enterococcus avium, Enterococcus durans, Enterococcus gallinarum, Enterococcus casseliflavus (two isolates of each), and Enterococcus raffinosus, Enterococcus mundtii, Enterococcus malodoratus, and Enterococcus hirae (one isolate of each). For all isolates, species identification by biochemical testing was corroborated by 16S rRNA gene sequencing. The sequence of domain V of the 23S rRNA gene from E. faecium and E. faecalis differed from those of all other enterococci. The domain V sequences of E. durans and E. hirae were identical. This was also true for E. gallinarum and E. casseliflavus. E. avium differed from E. casseliflavus by 23 bases, from E. durans by 16 bases, and from E. malodoratus by 2 bases. E. avium differed from E. raffinosus by one base. Despite the fact that domain V is considered to be highly conserved, substantial differences were identified between several enterococcal species.
URL: 20514229
Ref #: 2848
Author(s): Collins,M.D.;Jones,D.;Farrow,J.A.E.;Kilpper-Bälz,R.;Schleifer,K.H.
Journal: Int. J. Syst. Bacteriol.
Title: Enterococcus avium nom. rev., comb. nov.; E. casseliflavus nom. rev., comb. nov.; E. durans nom. rev., comb. nov.; E. gallinarum comb. nov.; and E. malodoratus sp. nov.
Volume: 34
Page(s): 220-223
Year: 1984
Ref #: 2851
Author(s): Farrow,J.A.E.;Jones,D.;Phillips,B.A.;Collins,M.D.
Journal: J. Gen. Microbiol.
Title: Taxonomic studies on some group D streptococci.
Volume: 129
Page(s): 1423-1432
Year: 1983
Ref #: 2852
Author(s): Kilpper-Bälz,R.;Fischer,G.;Schleifer,K.H.
Journal: Curr. Microbiol.
Title: Nucleic acid hybridization of group N and group D streptococci.
Volume: 7
Page(s): 245-250
Year: 1982
Ref #: 2853
Author(s): Sherman,J.M.;Wing,H.U.
Journal: J. Dairy Sci.
Title: Streptococcus durans nov. sp.
Volume: 20
Page(s): 165-167
Year: 1937
Data: (ATCC 19432, NCDO 596) Type strain / R. E. O. Williams, PHLS Colindale in 1950 / P. M. F. Shattock, NIRD Shinfield / Preparation of group D antiserum / Sherman, J. M. & Wing, H. U. (1937) J. Dairy Sci. 20, 165 / Shattock, P. M. F. (1949) J.gen.Microbiol. 3, 80
Accession Date: 01/01/1950
History: ISOLATED BY SHERMAN - SHATTOCK P M F NIRD SHINFIELD
Authority: Collins et al. 1984
Depositor: WILLIAMS R E O
Taxonomy: TaxLink: S1143 (Streptococcus durans) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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