Culture Collections

Bacteria and Mycoplasmas detail

Conditions of Supply of Microbial Pathogens: Safety





Bacteria Collection: Citrobacter freundii

NCTC Number: NCTC 9750
Current Name: Citrobacter freundii
Original Strain Reference: ATCC 8090
Other Collection No: ATCC 8090; DSM 30039; IFO 12681
Previous Catalogue Name: Citrobacter freundii
Type Strain: Yes
Family: Enterobacteriaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Conditions for growth on solid media: Nutrient agar, 24 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS485849
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|AJ233408|DSM 30039|Citrobacter freundii 16S rRNA gene (strain DSM 30039).| ggctcagattgaacg...
23S rRNA Gene Sequence: >gb|AJ549510|TYPE STRAIN: DSM 30039|Citrobacter freundii partial 23S rRNA gene, type strain DSM 30039T.| tttaaagaaagcgta...
Miscellaneous Sequence Data: >gb|AJ300528|CIP 57.32T| ATCC 8090|Citrobacter freundii partial gyrB gene for DNA gyrase B subunitstrain CIP 57.32T, ATCC 8090.| ataagtttgatgaca...
Bibliography: JUDICIAL COMMISSION 1964 INT BULL BACT NOM- ENCL TAXON 13 14 ; JUCICIAL
Extended Bibliography: showhide Show bibliography
Ref #: 45324
Author(s): Sproer,C.;Mendrock,U.;Swiderski,J.;Lang,E.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae
Volume: 49 Pt 4
Page(s): 1433-8
Year: 1999
Keyword(s): GENBANK/AJ233400 GENBANK/AJ233401 GENBANK/AJ233402 GENBANK/AJ233403 GENBANK/AJ233404 GENBANK/AJ233405 GENBANK/AJ233406 GENBANK/AJ233407 GENBANK/AJ233408 GENBANK/AJ233409 GENBANK/AJ233410 GENBANK/AJ233411 GENBANK/AJ233412 GENBANK/AJ233413 GENBANK/AJ233414 GENBANK/AJ233415 GENBANK/AJ233416 GENBANK/AJ233417 GENBANK/AJ233418 GENBANK/AJ233419 GENBANK/AJ233420 GENBANK/AJ233421 GENBANK/AJ233422 GENBANK/AJ233423 GENBANK/AJ233424 GENBANK/AJ233425 GENBANK/AJ233426 GENBANK/AJ233427 GENBANK/AJ233428 GENBANK/AJ233429 etc. DNA, Ribosomal/genetics Enterobacteriaceae/classification/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Serratia/classification/*genetics
Remarks: The phylogenetic relationships of the type strains of 38 species from 15 genera of the family Enterobacteriaceae were investigated by comparative 16S rDNA analysis. Several sequences of strains from the genera Citrobacter, Erwinia, Pantoea, Proteus, Rahnella and Serratia, analysed in this study, have been analysed previously. However, as the sequences of this study differ slightly from the published ones, they were included in the analysis. Of the 23 enterobacterial genera included in an overview dendrogram of relatedness, members of the genera Xenorhabdus, Photorhabdus, Proteus and Plesiomonas were used as a root. The other genera formed two groups which could be separated, although not exclusively, by signature nucleotides at positions 590-649 and 600-638. Group A contains species of Brenneria, Buttiauxella, Citrobacter, Escherichia, Erwinia, Klebsiella, Pantoea, Pectobacterium and Salmonella. All seven type strains of Buttiauxella share 16S rDNA similarities greater than 99%. Group B embraces two phylogenetically separate Serratia clusters, a lineage containing Yersinia species, Rahnella aquatica, Ewingella americana, and also the highly related pair Hafnia alvei and Obesumbacterium proteus.
URL: 10555323
Ref #: 95479
Author(s): Mitterer,G.;Huber,M.;Leidinger,E.;Kirisits,C.;Lubitz,W.;Mueller,M.W.;Schmidt,W.M.
Journal: J Clin Microbiol
Title: Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences
Volume: 42
Page(s): 1048-57
Year: 2004
Keyword(s): Abortion, Veterinary/microbiology Animals Bacteria/*genetics/*isolation & purification Base Sequence DNA Primers DNA, Bacterial/genetics/isolation & purification DNA, Ribosomal/*genetics/isolation & purification Female Horses *Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction/*methods Pregnancy Pregnancy Complications, Infectious/microbiology/veterinary RNA, Bacterial/genetics/isolation & purification RNA, Ribosomal, 23S/*genetics/isolation & purification
Remarks: The rapid identification of the bacteria in clinical samples is important for patient management and antimicrobial therapy. We describe a DNA microarray-based PCR approach for the quick detection and identification of bacteria from cervical swab specimens from mares. This on-chip PCR method combines the amplification of a variable region of bacterial 23S ribosomal DNA and the simultaneous sequence-specific detection on a solid phase. The solid phase contains bacterial species-specific primers covalently bound to a glass support. During the solid-phase amplification reaction the polymerase elongates perfectly matched primers and incorporates biotin-labeled nucleotides. The reaction products are visualized by streptavidin-cyanine 5 staining, followed by fluorescence scanning. This procedure successfully identified from pure cultures 22 bacteria that are common causes of abortion and sterility in mares. Using the on-chip PCR method, we also tested 21 cervical swab specimens from mares for the presence of pathogenic bacteria and compared the results with those of conventional bacteriological culture methods. Our method correctly identified the bacteria in 12 cervical swab samples, 8 of which contained more than one bacterial species. Due to the higher sensitivity of the on-chip PCR, this method identified bacteria in five cervical swab samples which were not detected by the conventional identification procedure. Our results show that this method will have great potential to be incorporated into the routine microbiology laboratory.
URL: 15004052
Ref #: 48709
Author(s): Dauga,C.
Journal: Int J Syst Evol Microbiol
Title: Evolution of the gyrB gene and the molecular phylogeny of Enterobacteriaceae: a model molecule for molecular systematic studies
Volume: 52
Page(s): 531-47
Year: 2002
Keyword(s): GENBANK/AJ300528 GENBANK/AJ300529 GENBANK/AJ300530 GENBANK/AJ300531 GENBANK/AJ300532 GENBANK/AJ300533 GENBANK/AJ300534 GENBANK/AJ300535 GENBANK/AJ300536 GENBANK/AJ300537 GENBANK/AJ300538 GENBANK/AJ300539 GENBANK/AJ300540 GENBANK/AJ300541 GENBANK/AJ300542 GENBANK/AJ300543 GENBANK/AJ300544 GENBANK/AJ300545 GENBANK/AJ300546 GENBANK/AJ300547 GENBANK/AJ300548 GENBANK/AJ300549 GENBANK/AJ300550 GENBANK/AJ300551 GENBANK/AJ300552 GENBANK/AJ300553 GENBANK/AJ300554 DNA Gyrase/*genetics Enterobacteriaceae/*classification/genetics Evolution, Molecular Genes, rRNA Molecular Sequence Data Phenotype RNA, Bacterial/chemistry RNA, Ribosomal, 16S/chemistry
Remarks: Phylogenetic trees showing the evolutionary relatedness of Enterobacteriaceae based upon gyrB and 16S rRNA genes were compared. Congruence among trees of these molecules indicates that the genomes of these species are not completely mosaic and that molecular systematic studies can be carried out. Phylogenetic trees based on gyrB sequences appeared to be more reliable at determining relationships among Serratia species than trees based on 16S rRNA gene sequences. gyrB sequences from Serratia species formed a monophyletic group validated by significant bootstrap values. Serratia fonticola had the most deeply branching gyrB sequence in the Serratia monophyletic group, which was consistent with its atypical phenotypic characteristics. Klebsiella and Enterobacter genera seemed to be polyphyletic, but the branching patterns of gyrB and 16S rRNA gene trees were not congruent. Enterobacter aerogenes was grouped with Klebsiella pneumoniae on the gyrB phylogenetic tree, which supports that this species could be transferred to the Klebsiella genus. Unfortunately, 16S rRNA and gyrB phylogenetic trees gave conflicting evolutionary relationships for Citrobacter freundii because of its unusual gyrB evolutionary process. gyrB lateral gene transfer was suspected for Hafnia alvei. Saturation of gyrB genes was observed by the pairwise comparison of Proteus spp., Providencia alcalifaciens and Morganella morganii sequences. Depending on their level of variability, 16S rRNA gene sequences were useful for describing phylogenetic relationships between distantly related Enterobacteriaceae, whereas gyrB sequence comparison was useful for inferring intra- and some intergeneric relationships.
URL: 11931166
Ref #: 12300
Author(s): Sproer,C.;Mendrock,U.;Swiderski,J.;Lang,E.;Stackebrandt,E.
Journal: Int J Syst Bacteriol
Title: The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae
Volume: 49 Pt 4
Page(s): 1433-8
Year: 1999
Keyword(s): GENBANK/AJ233400 GENBANK/AJ233401 GENBANK/AJ233402 GENBANK/AJ233403 GENBANK/AJ233404 GENBANK/AJ233405 GENBANK/AJ233406 GENBANK/AJ233407 GENBANK/AJ233408 GENBANK/AJ233409 GENBANK/AJ233410 GENBANK/AJ233411 GENBANK/AJ233412 GENBANK/AJ233413 GENBANK/AJ233414 GENBANK/AJ233415 GENBANK/AJ233416 GENBANK/AJ233417 GENBANK/AJ233418 GENBANK/AJ233419 GENBANK/AJ233420 GENBANK/AJ233421 GENBANK/AJ233422 GENBANK/AJ233423 GENBANK/AJ233424 GENBANK/AJ233425 GENBANK/AJ233426 GENBANK/AJ233427 GENBANK/AJ233428 GENBANK/AJ233429 etc. DNA, Ribosomal/genetics Enterobacteriaceae/classification/*genetics Molecular Sequence Data *Phylogeny Polymerase Chain Reaction/methods RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Serratia/classification/*genetics
Remarks: The phylogenetic relationships of the type strains of 38 species from 15 genera of the family Enterobacteriaceae were investigated by comparative 16S rDNA analysis. Several sequences of strains from the genera Citrobacter, Erwinia, Pantoea, Proteus, Rahnella and Serratia, analysed in this study, have been analysed previously. However, as the sequences of this study differ slightly from the published ones, they were included in the analysis. Of the 23 enterobacterial genera included in an overview dendrogram of relatedness, members of the genera Xenorhabdus, Photorhabdus, Proteus and Plesiomonas were used as a root. The other genera formed two groups which could be separated, although not exclusively, by signature nucleotides at positions 590-649 and 600-638. Group A contains species of Brenneria, Buttiauxella, Citrobacter, Escherichia, Erwinia, Klebsiella, Pantoea, Pectobacterium and Salmonella. All seven type strains of Buttiauxella share 16S rDNA similarities greater than 99%. Group B embraces two phylogenetically separate Serratia clusters, a lineage containing Yersinia species, Rahnella aquatica, Ewingella americana, and also the highly related pair Hafnia alvei and Obesumbacterium proteus.
URL: 20023023
Ref #: 1300
Author(s): Skerman,V.B.D.;McGowan,V.;Sneath,P.H.A.(ed)
Journal: Int. J. Syst. Bacteriol.
Title: Approved Lists of Bacterial Names.
Volume: 30
Page(s): 225-420
Year: 1980
Ref #: 4300
Author(s): Jagnow,G.;Haider,K.;Ellwardt,P.-C.
Journal: Arch. Microbiol.
Title: Anaerobic dechlorination and degradation of hexachlorocyclohexane isomers by anaerobic and facultative anaerobic bacteria.
Volume: 115
Page(s): 285-292
Year: 1977
Ref #: 6924
Author(s): DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed)
Title: DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme.
Year: 1997
Data: Type strain / ATCC in 1953 / Judicial Commission (1963) Int. Bull. Bact. Nomencl. Taxon. 13, 35 and (1964) 14, 59
Accession Date: 01/01/1953
Authority: (Braak 1928) Werkman and Gillen 1932 (AL)
Depositor: ATCC
Taxonomy: TaxLink: S800 (Citrobacter freundii (Braak 1928) Werkman and Gillen 1932) - Date of change: 5/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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The Culture Collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the Collection. Nevertheless, some of the data we provide about the cultures is supplied by the person depositing the strains and, although we have multiple checking procedures in place, we cannot always verify all their data. Please note that the Culture Collections cannot be held responsible for any inaccuracies in the data provided by the depositors.

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