Culture Collections

Cell Line Authentication & Quality Control Information

 

All cell lines supplied by ECACC undergo comprehensive quality control and authentication procedures. These include testing for mycoplasma by culture isolation, Hoechst DNA staining and PCR, together with culture testing for contaminant bacteria, yeast and fungi.

Authentication procedures used include species verification by DNA barcoding and identity verification by DNA profiling. Human cell lines are analysed by PCR of short tandem repeat sequences within chromosomal microsatellite DNA (STR-PCR).

Certificates of Analysis are available on request for each cell line lot no. supplied which report the viable cell count, identity of the cell line and the absence of bacteria, fungi and mycoplasma.

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