Culture Collections

Cell line depositor: Imperial College London

 Imperial College London

 

 

Imperial College London is a global top ten university with a world-class reputation in science, engineering, business and medicine. Imperial is the only UK university to focus entirely on science, engineering, medicine and business, and is the most innovative university in the UK and 2nd in Europe (Reuters, 2019).

 

Portfolio of human colorectal adenocarcenoma

Imperial College London have deposited a portfolio of human colorectal adenocarcenoma cell lines with ECACC which includes primary cell lines isolated from patients (of various Duke's stages and histological grades) as well as cloned cells lines genetically modified to highly express alpha integrin domains.

 

Technology overview

Primary

Six cell lines from primary human colorectal adenocarcinomas were established from surgical specimens without the use of 'feeder' cells, 'conditioned' medium or passage of cells in nude mice. The six cell lines exhibit considerable variation in morphology, CEA secretion and tumourigenicity in nude mice. At least two of the lines retain some of the differentiated characteristics of colorectal epithelium. HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.

Clonal

HRA-19a1.1 is a clone derived from HRA-19. The HRA-19a1.1 cell line was transfected with alpha integrin domain coding plasmids to create cell lines highly expressing alpha2 or alpha2 and alpha 1 integrin. In experiments, cell lines expressing alpha2 showed a significantly increased endrocrine and mucous cell lineage commitment relative to the parent cell line.

Benefits
  • Cell lines are invaluable tools for biomedical research
  • Cancer cell lines are the foundation of cancer biology and the quest for drug treatments
  • Adenocarcinoma is a cancer of the epithelium (including colon) and originates in glandular tissue, such as skin surface layer and glands

 

 

 Cell Line Name

 Catalogue Number

 HCA-7 Colony 29   

 02091238                          

 HCA-2

 06061901

 HCA-7

 06061902

 HCA-24

 06061903

 HRA-16

 06061905

 HCA-46

 07031601

 HCA-7 Colony 1

 09071501

 HCA-7 Colony 3

 09071502

 HCA-7 Colony 6

 09071504

 HCA-7 Colony 11

 09071506

 HCA-7 Colony 24

 09071509

 HCA-7 Colony 26

 09071511

 HCA-7 Colony 27

 09071513

 HCA-7 Colony 30

 09071516

 HRA-19a1.1

 09071517

 HRA-19a1.1 Alpha 2F

 09071520

 HRA-19a1.1 Alpha2 B

 09071522

 HRA-19a1.1 Alpha2Alpha1 B

 09071524

 HRA-19a1.1 Alpha2Alpha1 E

 09071527

 HRA-19

 10012802

 

For more information, please contact culturecollections.businesE@phe.gov.uk 

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