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MOR/0.4R

MOR/0.4R

Catalogue No.

96042334

Cell Line Name

MOR/0.4R

Cell Line Description

MOR/0.4R has been developed from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). This drug-resistant cell line accumulates lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance-associated protein (MRP). The expression of a 190kDa membrane protein associated with the degree of drug resistance has been indicated. Cells grow as easily detaching aggregates

Characteristics

Tissue of Origin

Lung

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 10
D5S818: 12
D7S820: 9
D13S317: 12
D16S539: 9
TH01: 7
TPOX: 11
vWA: 15

Karyotype

Not specified

Applications

Analysis of acquired drug resistance, MRP studies

Disease

Adenocarcinoma

Culture Conditions

Subculture Routine

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a lot of cell debris.

Culture Medium

RPMI 1640 + 2mM Glutamine + 0.4µg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).

Growth Mode

Semi-adherent aggregates

Additional Info

Depositor

Dr P Twentyman, UKCCCR, Lincolns Inn Fields, London

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Natl Cancer Inst 1994;86:110; Int J Cancer 1995;63:855

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: MOR/0.4R (ECACC 96042334).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.