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UK Health Security Agency

MM253

MM253

Catalogue No.

11072804

Cell Line Name

MM253

Cell Line Description

MM253 is a human melanoma derived from a metastatic site (lymph node). This cell line is distributed by ECACC on behalf of CellBank Australia, Children's Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by CellBank Australia. CellBank Australia reference number CBA-1347

Characteristics

Tissue of Origin

Skin

Disease

Melanoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Optimal split ratio 1:4 - 1:8 (seeding density 0.8 x10⁴ cells/cm²).Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Culture Medium

RPMI 1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FBS

Growth Mode

Adherent

Additional Info

Depositor

Peter Parsons - Queensland Institute of Medical Research, Australia

Country of Origin

Australia

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Parsons PG, Morrison L. 1978 Melphalan-induced chromosome damage in sensitive and resistant human melanoma cell lines. Int J Cancer. 21(4):438-43 PMID: 669848.

Bibliography

Castellano M, Pollock PM, Walters MK, Sparrow LE, Down LM, Gabrielli BG, Parsons PG, Hayward NK. 1997 CDKN2A/p16 is inactivated in most melanoma cell lines. Cancer Res. 57(21):4868-75 PMID: 9354451. Pavey S, Johansson P, Packer L, Taylor J, Stark M, Pollock PM, Walker GJ, Boyle GM, Harper U, Cozzi SJ, Hansen K, Yudt L, Schmidt C, Hersey P, Ellem KA, O'Rourke MG, Parsons PG, Meltzer P, Ringnér M, Hayward NK. 2004 Microarray expression profiling in melanoma reveals a BRAF mutation signature. Oncogene. 23(23):4060-7 PMID: 15048078. Stark M, Hayward N. 2007 Genome-wide loss of heterozygosity and copy number analysis in melanoma using high-density single-nucleotide polymorphism arrays. Cancer Res. 67(6):2632-42 PMID: 17363583.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: MM253 (ECACC 11072804).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.