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UK Health Security Agency

LA-N-1

LA-N-1

Catalogue No.

06041201

Cell Line Name

LA-N-1

Cell Line Description

Established by Seeger et al., (1977) from neuroblastoma cells in the bone marrow of a 2-year-old male with clinical Stage IV neuroblastoma. The cells are neuroblastic, tear-drop shaped with multiple short fine cell processes (neurites). The cells are tumourigenic in nude mice. This is a catecholamine producing neuroblastoma cell line.LA-N-1 (ECACC Catalogue number 06041201), LA1-55n (ECACC Catalogue number 06041203) and LA1-5s (ECACC Catalogue number 06041204) have been shown to originate from the same patient by STR profiling.

Characteristics

Products

Catecholamine

Tissue of Origin

Neuroblastoma

Morphology

Tear-drop shaped

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 12
D5S818: 12
D7S820: 10,11
D13S317: 11,12
D16S539: 9
TH01: 8,9.3
TPOX: 8,11
vWA: 16,19

Karyotype

Modal no. 87, range 47 - 87

Disease

Neuroblastoma

Culture Conditions

Cell Type

Neuronal

Subculture Routine

For routine maintenance, split cultures only after they have become very dense i.e. split once every 2-3 weeks at a 1:20 - 1:100 ratio; 8% CO₂; 37°C. Cells grow best and are most adherent on a plastic substrate in medium at a pH of 6.9 - 7.2; they do not tolerate more alkaline pH well. Population doubling time is approximately 2 days; saturation density is >1,000,000 cells/cm². Cells grow in weakly adherent clusters. Allow all floating clumps to settle and withdraw most of the medium. Remove attached cells from substrate with 0.1% trypsin or PBS alone, cells will detach in <5 minutes. Remaining cells, if any, are large, flat and tightly adherent precursors of Schwann cell/glia/melanocyte lineages. If desired they should be removed from the substrate with trypsin/EDTA. When the neuronal cells are seeded into a new flask the cells attach slowly and may remain in suspension for one to several days; do not change the medium the day after passage. Floating clumps of cells are viable. This passage schedule will select for cells retaining a neuroblastic phenotype; cultures transferred more frequently, with larger inocula, and/or with trypsin/EDTA may gradually lose their neuroblastic properties due to overgrowth by spontaneously arising, non-neuronal adherent cell variants. When cells are resuscitated from a frozen ampoule the cells may appear dead after a day, but reattach and resume growth within 2-3 days reaching confluence by 7 days.

Culture Medium

EMEM (with non-essential amino acids) and Ham's F12 (1:1 mixture) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Semi-adherent aggregates

Additional Info

Depositor

Dr Robert A Ross, Laboratory of Neurobiology, Department of Biological Sciences, Fordham University, New York, USA

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Seeger RC, Rayner SA, Banerjee A, Chung H, Laug WE, Neustein HB, Benedict WF 1997 Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines. Cancer Res. 37(5):1364-71 PMID: 856461

Bibliography

Ross RA, Biedler JL, Spengler BA, Reis DJ 1981 Neurotransmitter-synthesizing enzymes in 14 human neuroblastoma cell lines. Cell Mol Neurobiol. 1(3):301-11 PMID: 6125265

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: LA-N-1 (ECACC 06041201).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.