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UK Health Security Agency

HCA-7

HCA-7

Catalogue No.

06061902

Cell Line Name

HCA-7

Cell Line Description

Established from a colon adenocarcinoma of a 58 year-old female patient. The adenocarcinoma was moderately differentiated and determined to be Dukes' stage B. Imperial College Innovations has stated that this cell line is not to be supplied to commercial organisations.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Colon

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,14
D5S818: 9,12
D7S820: 11,12
D13S317: 9
D16S539: 10,11
TH01: 6,7
TPOX: 8,10
vWA: 14,16

Karyotype

Not specified

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:10 using 0.05% trypsin/EDTA; 7.5% CO₂; 37°C. Suggested seeding density 2-4x10,000 cells/cm².

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road, London, W12 0NN

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

S. C. Kirkland and I. G. Bailey. (1986) Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer, 53, 6, 779-85.

Bibliography

Ilyas et al., (1997). Beta-catenin mutations in cell lines established from human colorectal cancers. PNAS 94: 10330-10334. Wheeler et al., (1999). Mechanisms of inactivation of mismatch repair genes in human colorectal cancer cell lines: The predominant role of hMLH1. PNAS 96: 10296-10301. Bicknell & Bodmer, (2005). A mutated HLA-A*0101 allele in the colorectal cell line HCA-7. Tissue Antigens 66: 231-237. Liu & Bodmer, (2006). Analysis of P53 mutations and their expression in 56 colorectal cancer cell lines. PNAS 103: 976-981.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: HCA-7 (ECACC 06061902).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.