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UM-UC-1

UM-UC-1

Catalogue No.

06080301

Cell Line Name

UM-UC-1

Cell Line Description

Human transitional cell carcinoma (grade II) derived from a bladder cancer metastasis of a black male. Metastatic involvement of lymphatics. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Bladder

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12
D5S818: 13
D7S820: 8,11
D13S317: 12,14
D16S539: 9,11
TH01: 8
TPOX: 8
vWA: 14,15

Karyotype

Not specified

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time approximately 26 hours.

Culture Medium

MEM + 1% NEAA + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr H Barton Grossman, Department of Urology, University of Texas, M.D. Anderson Cancer Center, 1515 Holcombe Boulevard, Texas 77030 USA

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Grossman et al., (1984) UM-UC-1 and UM-UC-2: characterization of two new human transitional cell carcinoma lines. Journal of Urology 132: 834-837

Bibliography

Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: UM-UC-1 (ECACC 06080301).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.