Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: KARPAS 417

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 06100302
Cell Line Name: KARPAS 417
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: KARPAS 417 (ECACC 06100302)
Keywords: Human myeloma
Cell Line Description: Established from the peripheral blood of a 56 year-old male diagnosed with multiple myeloma.
Species: Human
Tissue of Origin: Myeloma
CellType: Single round to polymorphic cells growing in suspension
Growth Mode: Suspension
Karyotype: Complex translocations, refer to Karpas et al., 2005
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Maintain cultures between 0.5 - 2 x 1000,000 cells/ml; 5% CO2; 37°C. Doubling time approximately 60 hours. Split saturated culture 1:2 every 2-4 days. Round to polymorhpic cells growing singly or in small clusters in suspension.
Culture Medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Depositor: Dr Abraham Karpas, Department of Haematology, University of Cambridge, MRC Centre, Hills Road, Cambridge, UK
Originator: Yes
Country: UK
References: Karpas et al., (2005) Studies of four new human myeloma cell lines. Leukemia & Lymphoma 46(1): 101-112
Additional Bibliography: Not Available
Patents: None Specified By Depositor
Release Conditions: Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

Frozen

Formats Price
  • Frozen
    Re-stocking *
    £530.00
Quantity
*Re-stocking: contact for availability
Credit Account orders accepted or email / fax your order to us.






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