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UK Health Security Agency

B82 (Hamprecht)

B82 (Hamprecht)

Catalogue No.

08062522

Cell Line Name

B82 (Hamprecht)

Cell Line Description

An 8-bromodeoxyuridine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). B82 lacks thymidine kinase and was isolated by virtue of resistance to 8-bromodeoxyuridine (30 µg/ml). ECACC has two catalogue entries for the B82 cell line which differ in that they were deposited by different groups: B82 (ECACC catalogue number 85011408) and B82(Hamprecht) (ECACC catalogue number 08062522). Several other cell lines in the ECACC catalogue have been derived from B82(Hamprecht).

Characteristics

Tissue of Origin

Not specified

Karyotype

Modal No of chromosomes is 53

Applications

Used in the generation of cell hybrids

Disease

Neuroblastoma

Culture Conditions

Cell Type

Fibroblastic

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine

Growth Mode

Adherent

Additional Info

Depositor

Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.

Country of Origin

Germany

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Littlefield, (1966) The use of drug-resistant markers to study the hybridization of mouse fibroblasts. Exp. Cell Res. 41: 190-196.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: B82 (Hamprecht) (ECACC 08062522).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.