|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||HRA-19|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: HRA-19 (ECACC 10012802)|
|Keywords:||Human colorectal adenocarcinoma|
|Cell Line Description:||The HRA-19 cell line was established from a primary human rectal adenocarcinoma present in a 66-year old male. The adenocarcinoma was well differentiated and determined to be Dukes' stage B. A clonal derivative of HRA-19 is available (HRA-19a1.1, ECACC catalogue number 09071517). HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium. A number of genetically modified derivatives of HRA-19 are also available.|
|Tissue of Origin:||Rectum|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:5 to 1:10 weekly using 0.0125% trypsin/EDTA; 7.5% CO2; 37°C.Suggested seeding density 2-4x10,000 cells/cm².|
|Culture Medium:||DMEM (High glucose Sigma D6546) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr S Kirkland, Department of Histopathology, Imperial College London, Hammersmith Campus, DuCane Road London W12 0NN|
|References:||Kirkland & Bailey 1986 Establishment and characterisation of six human colorectal adenocarcinoma cell lines. Br J Cancer 53: 779-785. PMID: 3718830. Kirkland 1986 Endocrine differentiation by a human rectal adenocarcinoma cell line (HRA-19). Differentiation 33: 148-155. PMID: 3569697|
|Additional Bibliography:||Kirkland S C 1988 Clonal origin of columnar, mucous and endocrine cell lineages in human colorectal epithelium. Cancer 61: 1359-1363. PMID: 2449944. Henderson & Kirkland 1996 Multilineage differentiation of cloned HRA-19 cells inserum free medium: A model of human colorectal epithelial differentiation. Differentiation 60: 259-268. PMID: 8765056. Kirkland & Henderson 2001 Collagen IV synthesis is restricted to the enteroendocrine pathway during multilineage differentiation of human colorectal epithelial stem cells. Journal of Cell Science 114: 2055-2064. PMID: 11493641.|
|Patents:||None Specified By Depositor|
|Release Conditions:||Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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