|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||CMT 64|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: CMT 64 (ECACC 10032301)|
|Keywords:||Mouse lung carcinoma; metastasis|
|Cell Line Description:||CMT 64 was isolated from a primary alveogenic lung carcinoma tumour mass in C57BL/lcrf mouse, achieving stable morphology and growth rate in culture, similar to the growth rate and morphology in the primary tumour, and in lung metastasis induced after subcutaneous inoculation of mice. CMT 64 cells can be maintained in completely defined serum-free medium. Tumours derived from tissue culture cells grown in serum-free and serum-containing medium can form lung metastases in appropriate animal host.|
|Tissue of Origin:||Lung|
|CellType:||Epithelial, closely packed sheets at confluence|
|Karyotype:||Modal chromosome number 85|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density 1.2 x 105 cells/cm2|
|Culture Medium:||DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD|
|References:||Franks LM, Carbonell AW, Hemmings VJ, Riddle PN. (1976) Metastasizing tumors from serum-supplemented and serum-free cell lines from a C57BL mouse lung tumor. Cancer Res. Mar; 36(3):1049-55. PMID: 1253168.|
|Additional Bibliography:||Zhang K, Wrzesinski K, Fey SJ, Mose Larsen P, Zhang X, Roepstorff P. (2008) Assessing CMT cell line stability by two dimensional polyacrylamide gel electrophoresis and mass spectrometry based proteome analysis. J Proteomics. Jul 21; 71(2):160-7 PMID: 18617143. Patel AC, Anna CH, Foley JF, Stockton PS, Tyson FL, Barrett JC, Devereux TR. (2000) Hypermethylation of the p16 (Ink4a) promoter in B6C3F1 mouse primary lung adenocarcinomas and mouse lung cell lines. Carcinogenesis. Sep; 21(9):1691-700 PMID: 1096410. Maschek U, Pülm W, Hämmerling GJ. (1989) Altered regulation of MHC class I genes indifferent tumor cell lines is reflected by distinct sets of DNase I hypersensitive sites. EMBO J. Aug;8(8):2297-304. PMID: 2507317 . Smith GJ, Lykke AW. (1985) Characterization of a neoplastic epithelial cell strain derived by dexamethasone treatment of cultured normal mouse type 2 pneumocytes. J. Pathol. Nov; 147(3):165-72 PMID: 4067735. Franks LM, Layton MG. (1984) Ultrastructural tumour differentiation and organ specificity in high and low metastatic lines from a mouse lung carcinoma. Br J Cancer. Apr; 49(4):423-9 PMID: 6324837. Layton MG, Franks LM. (1984) Heterogeneity in a spontaneous mouse lung carcinoma: selection and characterisation of stable metastatic variants. Br J Cancer. Apr; 49(4):415-21 PMID: 6324836. Steele JG, Rowlatt C, Sandall JK, Franks LM. (1983) Cell surface properties of high- and low-metastatic cell lines selected from a spontaneous mouse lung carcinoma. Int J Cancer. Dec 15; 32(6):769-79 PMID: 6654529.|
|Release Conditions:||Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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