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TR146

TR146

Catalogue No.

10032305

Cell Line Name

TR146

Cell Line Description

TR146 was derived from the neck node of a 67 year-old female (primary tumour was sited in buccal mucosa). The patient had had previous radiotherapy (6,000 rads) and neck dissection. TR146 is tumourigenic in female (nu/nu) mice leading to the production of rapidly progressing tumours. Histology: well-differentiated keratinizing squamous cell carcinoma.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

Not specified

Products

Not specified

Tissue of Origin

Buccal mucosa

Morphology

Polygonal

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,13
D5S818: 10,11
D7S820: 10,12
D13S317: 11,14
D16S539: 13
TH01: 9
TPOX: 8,9
vWA: 15,18

Karyotype

Not specified

Applications

In vitro model of human buccal epithelial permeability

Disease

Squamous Cell Carcinoma

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) seeding at approximately 1-2 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells can reach a maximum density of 7 x 10⁴ cells/cm².

Culture Medium

HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Adherent

Additional Info

Depositor

Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Rupniak HT, Rowlatt C, Lane EB, Steele JG, Trejdosiewicz LK, Laskiewicz B, Povey S, Hill BT.(1985) Characteristics of four new human cell lines derived from squamous cell carcinomas of the head and neck. J Natl Cancer Inst. Oct; 75(4):621-35. PMID: 2413234.

Bibliography

Jacobsen J, Bjerregaard S, Pedersen M. (1999) Cyclodextrin inclusion complexes of antimycotics intended to act in the oral cavity--drug supersaturation, toxicity on TR146 cells and release from a delivery system. Eur J Pharm Biopharm. Nov; 48 (3):217-24. PMID: 10612032. Jacobsen J, Nielsen EB, Brøndum-Nielsen K, Christensen ME, Olin HB, Tommerup N, Rassing MR.(1999) Filter-grown TR146 cells as an in vitro model of human buccal epithelial permeability. Eur J Oral Sci. Apr; 107(2):138-46 PMID: 10232463. Pedersen M, Jacobsen J, Sørensen AM.(1999) Cyclodextrin inclusion complexes of miconazole and econazole--isolation, toxicity on human cells, and confirmation of a new interpretation of the drug supersaturation phenomenon. Drug Dev Ind Pharm. Apr; 25(4):463-70. PMID: 10194601.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: TR146 (ECACC 10032305).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.