Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: TR146

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 10032305
Cell Line Name: TR146
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: TR146 (ECACC 10032305)
Keywords: Human squamous cell carcinoma
Cell Line Description: TR146 was derived from the neck node of a 67 year-old female (primary tumour was sited in buccal mucosa). The patient had had previous radiotherapy (6,000 rads) and neck dissection. TR146 is tumourigenic in female (nu/nu) mice leading to the production of rapidly progressing tumours. Histology: well-differentiated keratinizing squamous cell carcinoma.
Species: Human
Tissue of Origin: Buccal mucosa
CellType: Polygonal
Growth Mode: Adherent
DNA Profile: STR-PCR Data:

Amelogenin: X
CSF1PO: 11,13
D13S317: 11,14
D16S539: 13
D5S818: 10,11
D7S820: 10,12
THO1: 9
TPOX: 8,9
vWA: 15,18

Karyotype: Not specified
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) seeding at approximately 1-2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells can reach a maximum density of  7 x 104 cells/cm2.
Culture Medium: HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Depositor: Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD
Originator: Yes
Country: UK
References: Rupniak HT, Rowlatt C, Lane EB, Steele JG, Trejdosiewicz LK, Laskiewicz B, Povey S, Hill BT.(1985) Characteristics of four new human cell lines derived from squamous cell carcinomas of the head and neck. J Natl Cancer Inst. Oct; 75(4):621-35. PMID: 2413234.
Additional Bibliography: Jacobsen J, Bjerregaard S, Pedersen M. (1999) Cyclodextrin inclusion complexes of antimycotics intended to act in the oral cavity--drug supersaturation, toxicity on TR146 cells and release from a delivery system. Eur J Pharm Biopharm. Nov; 48 (3):217-24. PMID: 10612032. Jacobsen J, Nielsen EB, Brøndum-Nielsen K, Christensen ME, Olin HB, Tommerup N, Rassing MR.(1999) Filter-grown TR146 cells as an in vitro model of human buccal epithelial permeability. Eur J Oral Sci. Apr; 107(2):138-46 PMID: 10232463. Pedersen M, Jacobsen J, Sørensen AM.(1999) Cyclodextrin inclusion complexes of miconazole and econazole--isolation, toxicity on human cells, and confirmation of a new interpretation of the drug supersaturation phenomenon. Drug Dev Ind Pharm. Apr; 25(4):463-70. PMID: 10194601.
Patents: None specified by depositor
Release Conditions: Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

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Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)
cDNA-20µl

Formats
  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

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