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UK Health Security Agency

MTSV1-7 CE1

MTSV1-7 CE1

Catalogue No.

10081202

Cell Line Name

MTSV1-7 CE1

Cell Line Description

MTSV1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7 transfected with ERBB2 expression vector, enabling the over-expression of ERBB2 proto-oncogene. Cultures of MTSV1-7 were transfected with the pSV2-erbB2 construct together with the pSV2neo plasmid. (See also 1-7HB2 ECACC Catalogue number 10081201) The MTSV1-7 CE1 cell line is an ideal tool for investigation of the role of the ERBB2 receptor proto-oncogene in breast cancer morphogenesis and adhesion. MTSV1-7 CE1 cells over-express the ERBB2 receptor, inducing a reduced ability to undergo morphogenesis in vitro, and reduced expression of surface adhesion molecules E-Cadherin and alpha-2 integrin. Inhibition of morphogenesis and transcription of adhesion molecules in human mammary epithelial cells can be affected by signals generated by the ERBB2 receptor, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Products

Formation of disorganised structures in collagen type I gels.

Tissue of Origin

Breast

Morphology

Cuboidal morphology at confluence

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 12,13
D5S818: 10,13
D7S820: 8,10
D13S317: 12
D16S539: 8,13
TH01: 7,9
TPOX: 11
vWA: 14,17

Disease

None Stated

Culture Conditions

Cell Type

Epithelial-like

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Hydrocortisone (5 µg/ml) + Bovine Insulin (10 µg/ml).

Growth Mode

Adherent

Additional Info

Depositor

Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Bartek J, Bartkova J, Kyprianou N, Lalani EN, Staskova Z, Shearer M, Chang S, Taylor-Papadimitriou J. (1991) Efficient immortalization of luminal epithelial cells from human mammary gland by introduction of simian virus 40 large tumor antigen with a recombinant retrovirus. Proc Natl Acad Sci U S A. 88(9):3520-4. PMID: 1708884.

Bibliography

D'Souza B, Berdichevsky F, Kyprianou N, Taylor-Papadimitriou J. (1993) Collagen-induced morphogenesis and expression of the alpha 2-integrin subunit is inhibited in c-erbB2-transfected human mammary epithelial cells. Oncogene. 8(7):1797-806. PMID: 8099725. D'Souza B & Taylor-Papadimitriou J. (1994) Overexpression of ERBB2 in human mammary epithelial cells signals inhibition of transcription of the E-cadherin gene. Proc Natl Acad Sci U S A. 91(15):7202-6. PMID: 7913748

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: MTSV1-7 CE1 (ECACC 10081202).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.