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JIM3

JIM3

Catalogue No.

10081204

Cell Line Name

JIM3

Cell Line Description

JIM3 is a myeloma tumour line demonstrating typical plasma cell phenotype (CD38, PCA-1 8A and CD24 positive), deficient in DNA repair. JIM3 was derived from plasma myeloma cells derived from pleural fluid of advanced multiple myeloma patient, homoplastic with JIM1. Used for in vitro study and comparison of genetic instability in myeloma tumour lines (homoplastic with JIM1) and the in vitro study of effects of DNA repair deficiency in myeloma tumour lines

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Blood (pleural effusion)

Morphology

Small spherical

Disease

Myeloma

Culture Conditions

Cell Type

Haematopoietic

Subculture Routine

Seed at 2-9 x 10⁵ cells/cm³ 5% CO₂; 37°C.

Culture Medium

Dexter culture medium, consisting of Fischer's medium + 20% FCS +10⁻⁷ M hydrocortisone sodium succinate

Growth Mode

Suspension

Additional Info

Depositor

Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Hamilton MS, Barker HF, Ball J, Drew M, Abbot SD, Franklin IM. (1991) Normal and neoplastic human plasma cells express bcl-2 antigen. Leukemia.5(9):768-71 PMID: 1943229.

Bibliography

Velangi MR, Matheson EC, Morgan GJ, Jackson GH, Taylor PR, Hall AG, Irving JA. (2004) DNA mismatch repair pathway defects in the pathogenesis and evolution of myeloma. Carcinogenesis. 25(10):1795-803 PMID: 15142887

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: JIM3 (ECACC 10081204).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.