Catalogue No.
11012007
SK-GT-4
SK-GT-4
Cell Line Name
SK-GT-4
Cell Line Description
SK-GT-4 was established from a primary tumour in 1989 from a 89 year-old Caucasian male who presented with dysphagia secondary to a well-differentiated adenocarcinoma arising in the Barrett epithelium of the distal oesophagus. The tumour invaded into but not through the muscle layer (T2) and involved 3 of 14 lymph nodes (N1). SK-GT-4 was found to be tumorigenic in athymic nu/nu mice.Cultures derived from ECACC stocks of this cell line have been whole genome sequenced (Contino et al 2016) confirming the presence of many of the known mutations that drive oesophageal cancer
General Info
Species
Human
Release Conditions
Characteristics
Receptors
Not specified
Products
Expression of actin imtermediate filaments and growth factors TGFβ1, TGFβ2, TGFβ3, TGFα and platelet-derived growth factor A (PDGFA). P53 mutation c.298C>T
Tissue of Origin
Distal Oesophagus
Karyotype
Modal number 59 chromosomal aberration at 11p13-15.
Disease
Adenocarcinoma
Culture Conditions
Cell Type
Epithelial growing in islands
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cell population doubling time 39 hours.
Culture Medium
RPMI-1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Growth Mode
Adherent
Additional Info
Depositor
Dr W.N.M. Dinjens, Department of Pathology, Josephine Nefkens Institute, Erasmus MC, University Medical Centre, Rotterdam PO Box 2040, 3000CA Rotterdam. Originator: Prof N Altorki Weill Cornell Medical Centre, 525 East 69th Street Box 110 New York NY 10065.
Country of Origin
United States
Hazard Group (ACDP)
Hazard Group (ACDP) 2
Applications
References
Altorki N, Schwartz GK, Blundell M, Davis BM, Kelsen DP, Albino AP. 1993 Characterization of cell lines established from human gastric-esophageal adenocarcinomas. Biologic phenotype and invasion potential. Cancer.72 (3):649-57 PMID: 8334620. Rodriguez E, Rao PH, Ladanyi M, Altorki N, Albino AP, Kelsen DP, Jhanwar SC, Chaganti RS.1990 11p13-15 is a specific region of chromosomal rearrangement in gastric and esophageal adenocarcinomas. Cancer Res. 50(19):6410-6 PMID: 2400998. Schwartz GK, Jiang J, Kelsen D, Albino AP. 1993 Protein kinase C: a novel target for inhibiting gastric cancer cell invasion. J Natl Cancer Inst. 85 (5):402-7 PMID: 8433394.Whole-genome sequencing of nine esophageal adenocarcinoma cell lines
Bibliography
Boonstra JJ, van Marion R, Beer DG, Lin L, Chaves P, Ribeiro C, Pereira AD, Roque L, Darnton SJ, Altorki NK, Schrump DS, Klimstra DS, Tang LH, Eshleman JR, Alvarez H, Shimada Y, van Dekken H, Tilanus HW, Dinjens WN. 2010 Verification and unmasking of widely used human esophageal adenocarcinoma cell lines. J Natl Cancer Inst. 102(4):271-4.PMID: 20075370.
Documents
Images
Available Formats
- Frozen
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: SK-GT-4 (ECACC 11012007).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.