Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: U3A

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 12021503
Cell Line Name: U3A
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: U3A (ECACC 12021503)
Keywords: alpha interferon signalling pathway, IFN regulated human gene 6-16, STAT1 gene product
Cell Line Description: The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The U3A mutant cell line lacks expression of STAT1 gene product. U3A is unresponsive to interferon-alpha and interferon-gamma. U3A is partially defective in its response to double stranded RNA.
Species: Human
Tissue of Origin: Fibrosarcoma
CellType: Epithelial
DNA Profile: STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

GMO Status: Genetically Modified Organism Class 1 (GMO1)
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Depositor: Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD. Originator: Dr GR Stark Imperial cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX
Originator: No
Country: UK
References: References on transfection: Blochlinger K, Diggelmann H.1984 Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells. Mol Cell Biol. 4(12):2929-31. PMID: 6098829. Kelly JM, Porter AC, Chernajovsky Y, Gilbert CS, Stark GR, Kerr IM.1986 Characterization of a human gene inducible by alpha- and beta-interferons and its expression in mouse cells. EMBO J. 5(7):1601-6. PMID: 3017706. Porter AC, Chernajovsky Y, Dale TC, Gilbert CS, Stark GR, Kerr IM.1988 Interferon response element of the human gene 6-16. EMBO J. 7(1):85-92. PMID: 3359997
Additional Bibliography: McKendry R, John J, Flavell D, Müller M, Kerr IM, Stark GR. 1991 High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons. Proc Natl Acad Sci U S A.; 88(24):11455-9. PMID: 1837150. Rani MR, Foster GR, Leung S, Leaman D, Stark GR, Ransohoff RM.1996 Characterization of beta-R1, a gene that is selectively induced by interferon beta (IFN-beta) compared with IFN-alpha. J Biol Chem.; 271(37):22878-84. PMID: 8798467
Patents: None specified by Depositor
Release Conditions: Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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