|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||293T|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: 293T (ECACC 12022001)|
|Keywords:||Human embryonic kidney SV40 transformed, genetically modified|
|Cell Line Description:||The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.|
|Tissue of Origin:||Kidney (foetal)|
|CellType:||Small slightly rounded cells growing in a dispersed manner|
|GMO Status:||Genetically Modified Organism Class 1 (GMO1)|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 104 cells/cm2|
|Culture Medium:||DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)|
|Depositor:||Professor Michele Calos, Department of Genetics, Alway Building, Room M-334 Stanford University School of Medicine 300 Pasteur Drive Stanford, CA 94305-5120.|
|References:||DuBridge RB, Tang P, Hsia HC, Leong PM, Miller JH, Calos MP. 1987 Analysis of mutation in human cells by using an Epstein-Barr virus shuttle system. Mol Cell Biol. 7(1):379-87.PMID: 3031469.|
|Additional Bibliography:||Pear WS, Nolan GP, Scott ML, Baltimore D. 1993 Production of high-titer helper-free retroviruses by transient transfection. Proc Natl Acad Sci U S A. 90(18):8392-6. PMID: 7690960. Sena-Esteves M, Saeki Y, Camp SM, Chiocca EA, Breakefield XO. 1999 Single-step conversion of cells to retrovirus vector producers with herpes simplex virus-Epstein-Barr virus hybrid amplicons. J Virol. 73(12):10426-39.PMID: 10559361.|
|Patents:||Pensiero M, et al. Retroviral vectors produced by producer cell lines resistant to lysis by human serum. US Patent 5,952,225 dated Sep 14 1999|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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