|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||ULK2 KO MEF (SIM)|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: ULK2 KO MEF (SIM) (ECACC 14050810)|
|Keywords:||Mouse embryonic fibroblast, autophagy, Unc51-like kinase, spontaneuosly immortalised|
|Cell Line Description:||Mouse embryonic fibroblasts (MEFs) derived from ULK1(+/-),ULK2(+/-) knockout mice. MEFs that were isolated and cultured were immortalised by standard serial passaging protocol. This cell line can be used to study ULK2-dependent processes in autophagy.|
|Species:||Mouse, mixed genetic background (Blk6/129 Agouti)|
|Tissue of Origin:||Embryo|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 104 per cm2. Cultures must be incubated in a humidified 5% CO2/95% air incubator at 37◦C.|
|Culture Medium:||DMEM + 10% FCS + 2mM Glutamine|
|Depositor:||Cancer Research Technology Limited|
|References:||McAlpine F, Williamson LE, Tooze SA, Chan EY. Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2. Autophagy 2013 Mar 9(3):361-73 PMID: 23291478|
|Additional Bibliography:||Chan EY, Longatti A, McKnight NC, Tooze SA. Kinase-inactivated ULK proteins inhibit autophagy via their onserved C-terminal domains using an Atg13-independent mechanism. Mol Cell Biol. 2009 Jan;29(1):157-71. Epub 2008 Oct 20. PubMed PMID: 18936157; PubMed Central PMCID: PMC2612494. Chan EY, Kir S, Tooze SA. siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem. 2007 Aug 31;282(35):25464-74. Epub 2007 Jun 26. PubMed PMID: 17595159. Young AR, Chan EY, Hu XW, Köchl R, Crawshaw SG, High S, Hailey DW, Lippincott-Schwartz J, Tooze SA. Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes. J Cell Sci. 2006 Sep 15;119(Pt 18):3888-900. Epub 2006 Aug 29. PubMed PMID: 16940348|
|Release Conditions:||Yes - commercial organisations are required to complete the ‘Cancer Research Technology Deposit Release Form for Commercial Organisations’|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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