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UK Health Security Agency

MEF RAF1 KO

MEF RAF1 KO

Catalogue No.

16012209

Cell Line Name

MEF RAF1 KO

Cell Line Description

The MEF RAF1 KO cell line is an immortalised mouse embryonic fibroblast line derived from a mouse containing a knockout Raf1 mutation. Mice carrying this mutation only survive to adulthood and normal developmental phenotype in specific genetic background, 129Ola/MF-1.

General Info

Species

Mouse

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Products

Knock-out of RAF-1 (raf-/-) from E14.5 days embryos in the 1290la/MF-1 background for the study of apoptosis and mouse development. The MEF RAF1 KO cell lines allows for drug screening toxicology studies in the absence if functional RAF1 gene.

Tissue of Origin

Embryo

Morphology

Fibroblast-like

Applications

Determination of the role of inactivating mutations and knockouts on the principal functions of the Raf protein kinases in the highly conserved Ras/Raf/MEK/ERK intracellular signalling pathway. The principal functions of the pathway includes activation by different classes of cell surface receptors including receptor tyrosine kinases (RTKs) and G protein coupled receptors which confer their biological effects through Ras. Raf-1 also functions in other processes including apoptosis and normal development in vivo

Disease

None Stated

Culture Conditions

Cell Type

Embryonic fibroblast

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10⁴ cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days

Culture Medium

Dulbecco's Modified Eagles medium with 10% FBS and 2mM L-Glutamine

Growth Mode

Adherent

Additional Info

Depositor

Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

Retroviral vector pZIPTEX, encoding both the SV40 large T-antigen gene and the bacterial neomycin resistance gene were based on the pZIP-Neo SV(X)1 vector

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Hüser M, Luckett J, Chiloeches A, Mercer K, Iwobi M, Giblett S, Sun XM, Brown J, Marais R, Pritchard C.MEK kinase activity is not necessary for Raf-1 function. EMBO J. 2001 Apr 17;20(8):1940-51. PMID: 11296227 [PubMed - indexed for MEDLINE]

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: MEF RAF1 KO (ECACC 16012209).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.