|Collection:||ECACC General Collection|
|Cell Line Name:||MCF7/182R-6|
|Keywords:||Breast cancer, fulvestrant resistant|
|Cell Line Description:||
The MCF7/182R-6 Cell line is a breast cancer cell line resistant to fulvestrant. The MCF7/182R-6 cell line has been established from a clone of MCF7/S0.5 cells surviving long term growth with the pure steroidal antiestrogen ICI 182,780 in 100 nM concentration, see Lykkesfeldt et al (1995). The MCF7/182R-6 cells are grown in the presence of fulvestrant.
Oestrogen receptor only. Upon withdrawal of fulvestrant, the resistant phenotype is stable and the cells express ER alpha, although at a reduced level. The MCF7/182R-6 cells do not express progesterone receptor. The MCF7/182R-6 cells express increased level of EGFR, phosphorylated EGFR and phosphorylated ErbB3 and reduced level of ErbB4 compared to the parental MCF7/S0.5 cells.
|Tissue of Origin:||Breast|
Amelogenin: X X
Penta E: 7,12
Penta D: 12,12
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x104 cells/cm² using 0.25% trypsin or trypsin or trypsin/EDTA; 5% CO2; 37°C.|
|Culture Medium:||Phenol red free DMEM/F12 (1:1) supplemented with 1% FCS, Glutamax 2.5 mM and 6 ng/ml insulin. Supplemented with 100nM fulvestrant to maintain resistance|
|Depositor:||Cancer Research Technologies Limited (Ximbio)|
|References:||Thrane et al. 2014. Oncogene. PMID: 25362855. Sonne-Hansen et al. 2010. Breast Cancer Res Treat. 121(3):601-13. PMID: 19697122. Frogne et al. 2009. Breast Cancer Res Treat. 114(2):263-75. PMID: 18409071. Frankel et al. 2007. Breast Cancer Res Treat. 104(2):165-79. PMID: 17061041. Frogne et al. 2005. Endocr Relat Cancer. 12(3):599-614. PMID: 16172194. Nabha et al. 2005. Oncogene. 24(19):3166-76. PMID: 15735693.|
|Additional Bibliography:||Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159.|
|Release Conditions:||Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' form|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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