|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||MCF7/AnaR-1|
|Organisation deposited by:||Ximbio (formerly known as Cancer Research Technologies Limited)|
|Unique to ECACC:||Yes|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: MCF7/AnaR-1 (ECACC 16022516)|
|Keywords:||Breast cancer, MCF7, Anastrozole resistance|
|Cell Line Description:||The MCF7/AnaR-1 cell line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. This breast cancer cell line is resistant to the aromatase inhibitor anastrozole and is oestrogen receptor positive. Derivation: Anastrozole-resistant cell lines were established from MCF7 cells grown in medium with 10% newborn calf serum (NCS) and 10−7 M testosterone. A culture of MCF7 cells were treated with 10−7 M anastrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with anastrozole. After ~2–3 months, the isolated colonies gave rise to anastrozole-resistant cell lines, which could be grown in anastrozole-containing medium with a weekly split ratio of ~1:25. The parental MCF7 cell line was authenticated in January 2014 by DNA profiling using short tandem repeat loci and found to be matching the genetic profile reported for the MCF7/AnaR-1 cell line.|
|Tissue of Origin:||Breast|
|Disease Status:||Breast cancer|
DNA Profile (STR Analysis):
Amelogenin: X X
CSF1PO: 10, 10
D13S317: 11, 11
D16S539: 11, 12
D21S11: 30, 30
D5S818: 11, 12
D7S820: 9, 9
D8S1179: 10, 14
FGA: 23, 25
Penta D: 12, 12
Penta E: 7, 12
TH01: 6, 6
TPOX: 9, 12 vWA: 14,15
|Hazard Group (ACDP):||2|
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
Split sub-confluent cultures at 70-80% confluency. Subculture cells using 0.05% trypsin/EDTA solution or TrypLE Express™ (1X), without phenol red, and wash with phosphate-buffered saline (PBS). Pellet cells by centrifugation and re-suspend in growth medium. Initiate the culture at a dilution of approximately 1:3 to 1:6 i.e. seeding at 2-4 x 104 cells per cm². Incubate cultures in 5% CO2 in air at 37°C. Recommend media changes every 2-3 days.
Cryopreserve cells in Newborn calf serum (90%) and dimethyl sulphoxide (10%). It is recommended that upon resuscitation the cells are washed in (PBS) or serum-free medium, pelleted by centrifugation and re-suspended in growth medium prior to initiation of culture.
|Culture Medium:||Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum (NCS), 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM anastrozole|
|Depositor:||Ximbio (formerly known as Cancer Research Technologies Limited)|
Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100
Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755
|Additional Bibliography:||Thewes et al. 2017. Oncogene. PMID: 28319069.|
|Release Conditions:||Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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