|Culture Type:||Cell Line|
|Collection:||ECACC General Collection|
|Cell Line Name:||MCF7/AnaR-4|
|Keywords:||Breast cancer, MCF7, Anastrozole resistance|
|Cell Line Description:||
The MCF7/AnaR-4 cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. This breast cancer cell line resistant to the aromatase inhibitor anastrozole and is oestrogen receptor positive.
Derivation: Anastrozole-resistant cell lines were established from MCF7 cells grown in medium with 10% newborn calf serum (NCS) and 10−7 Molar testosterone. A culture of MCF7 cells were treated with 10−7 Molar anastrozole for one week, trypsinized and seeded in serial dilutions in 24-well plates. Single colonies were transferred to new wells and gradually expanded in medium with anastrozole. After ~2-3 months, the isolated colonies gave rise to anastrozole-resistant cell lines, which could be grown in anastrozole-containing medium with a weekly split ratio of ~1:25.
|Tissue of Origin:||Breast|
Penta D: 12,12
Penta E: 7,12
Split sub-confluent cultures at 70-80% confluency. Subculture cells using 0.05% trypsin/EDTA solution or TrypLE Express™ (1X), without phenol red, and wash with phosphate- buffered saline (PBS). Pellet cells by centrifugation (~100 x g) and re-suspend in growth medium.
Initiate the culture at a dilution of approximately 1:3 to 1:6 i.e. seeding at 2-4 x 104 cells per cm2. Incubate cultures in 5% CO2 at 37°C. Recommend media changes every 2-3 days.
It is recommended that upon resuscitation the cells are washed with PBS or serum-free medium, pelleted by centrifugation and re-suspended in growth medium prior to initiation of culture.
|Culture Medium:||Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum (NCS), 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 0.1 uM anastrozole|
|Depositor:||Cancer Research Technologies Limited (Ximbio)|
Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100.
Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755
|Release Conditions:||Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' form|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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