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UK Health Security Agency

MEF OGG1 KO

MEF OGG1 KO

Catalogue No.

16022531

Cell Line Name

MEF OGG1 KO

Cell Line Description

Primary mouse embryo fibroblast (MEF) cultures from Ogg1 mutant mice were established by standard procedures from individual embryonic day 13.5 (E13.5) embryos derived from heterozygous matings. Cells were cultured in DMEM/Ham's F-12 (3:1) with 10% FBS and genotyped by Southern hybridization. Permanent cell lines were established from transformed clones arising spontaneously after repeated passage in culture.

General Info

Species

Mouse

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Embryo

Morphology

Fibroblast

Applications

The MEF OGG1 KO cell line can be used to assess the role of the mammalian enzyme OGG1 in repair of DNA damage and prevention of carcinogenesis. the cell line can be used to assess the incidence and consequences of 8-oxoG formation in the mammalian genome.

Disease

None Stated

Culture Conditions

Cell Type

Embryonic fibroblast

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10⁴ cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days

Culture Medium

DMEM/Ham's F-12 (3:1) with 10% FBS

Growth Mode

Adherent

Additional Info

Depositor

Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

United Kingdom

Genetic Modification

A highly conserved helix "hairpin" helix motif required for DNA glycosylase AP lyase activity was targeted for disruption. Approximately 4.6 kb of genomic DNA of the murine OGG1 gene was replaced by the neomycin resistance gene.

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: MEF OGG1 KO (ECACC 16022531).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.