Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: S_N40R2

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 17011210
Cell Line Name: S_N40R2
Organisation deposited by: Ximbio (formerly known as Cancer Research Technologies Limited)
Other Collection No.: No
Unique to ECACC: Yes
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: S_N40R2 (ECACC 17011210)
Keywords: MDM2 inhibitor, Nutlin-3, resistance, MDM2-application
Cell Line Description: S_N40R2 is a subpopulation of the SJSA-1 osteosarcoma line which is resistant to 40 uM Nutlin-3.
Species: Human
Tissue of Origin: Bone
Disease Status: Cancer, osteosarcoma, multipotential sarcoma
Donor Sex: Male
Donor Age: 19
Ethnic Origin: African American
CellType: Fibroblast
Morphology: Fibroblast
Growth Mode: Adherent
DNA Profile: DNA Profile (STR Analysis):

Amelogenin: X,Y

CSF1PO: 7.3, 10.1

D13S317: 12, 12

D16S539: 9, 10

D18S51: 15, 17

D21S11:28, 33.2

D3S1358: 16,17

D5S818: 12, 12

D7S820: 9, 11

D8S1179: 13, 15

FGA: 22, 23

Penta D 2.2, 10

Penta E: 11, 14

THO: 7, 9

TPOX: 8, 9

vWA: 19, 19

Genetic Modification: N/A
Hazard Group (ACDP): 2
Biosafety Information: Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance

Hyperlinks to Material Safety Data Sheets (MSDS) documents:
Frozen cell cultures MSDS
Growing cell cultures MSDS
Nucleic acids derived from cell cultures MSDS
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x104 cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO2, and passaged every 3 to 4 days

Freeze medium: Foetal Bovine Serum (FBS) 90% and DMSO 10%

Culture Medium: RPMI 1640+10% FBS
Depositor: Ximbio (formerly known as Cancer Research Technologies Limited)
Originator: Yes
Country: UK
References: Drummond CJ, Esfandiari A, Liu J, Lu X, Hutton C, Jackson J, Bennaceur K, Xu Q, Makimanejavali AR, Del Bello F, Piergentili A, Newell DR, Hardcastle IR, Griffin RJ, Lunec J.TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation. Oncotarget. 2016 Jul 19;7(29):46203-46218. doi: 10.18632/oncotarget PMID: 27323823
Cellosaurus Link: CVCL_HG10
Patents: None
Release Conditions: Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

Frozen
Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)
cDNA-20µl

Formats
  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

This product is only available through the depositor.







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