|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||HeLa EGFP-Histone H2B|
|Organisation deposited by:||Cancer Research Technologies Limited (Ximbio)|
|Unique to ECACC:||Yes|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: HeLa EGFP-Histone H2B (ECACC 17100529)|
|Keywords:||PP6 catalytic activity, EGFP, EGFP-tagged histone H2B|
|Cell Line Description:||The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression.|
|Tissue of Origin:||Cervix|
|Morphology:||Adherent epithelial cells|
DNA Profile (STR Analysis):
Amelogenin: X, X
|Hazard Group (ACDP):||2|
|Biosafety Information:||Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.|
Trypsinise cultures at 80% confluence with 0.05% Trypsin/EDTA. Seed flasks at 1-3 x104 cells/cm2. Cultures must be incubated in a humidified 10% CO2/90% air incubator at 37°C.
Note: The depositor had grown these cells at 5% CO2. Here at ECACC we grow cells at 10% CO2 when cells are grown in DMEM media.
|Culture Medium:||Dulbecco's Modified Eagle's Medium, high glucose (DMEM) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS). Recommend used of selection antibiotics (4μg/ml Blastocidin) for maintenance of expression. The transgene is stably integrated into the genome of the cells so for short term culture it is not strictly necessary to include the antibiotics.|
|Depositor:||Cancer Research Technologies Limited (Ximbio)|
|References:||Zeng., K. et al Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2. J Cell Biol. 2019 (Mar).191(7): 1315-1332. phttp://doi.org/10.1083/jcb.201008106. PMID: 21187329 PMCID: PMC3010072|
|Release Conditions:||Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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