|Supplied by:||CellBank Australia|
|Collection:||ECACC General Collection|
|Cell Line Name:||SIRMu-1|
|Cell Line Description:||
Spontaneously Immortalized Rat Müller cell line. Müller cells are a major type of glial cells in the retina of the eye. Strain: Sprague-Dawley
This cell line is distributed by ECACC on behalf of CellBank Australia, Children’s Medical Research Institute, Westmead, NSW 2145, Australia. All stock supplied is manufactured and Quality Control released at source by CellBank Australia.
|Species:||Rat (Rattus norvegicus)|
|Tissue of Origin:||Retina|
|GMO Status:||Genetically Modified Organism Class 1 (GMO1)|
|Hazard Group (ACDP):||1|
|Biosafety Information:||Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.|
Subcultivation Ratio: 1:5 - 1:10, split subconfluent cultures (70-80%). Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 minutes. At thaw, the cells should be seeded at a density of 2-3 x 104cells/cm2. After recovery from thaw (48-72 hours), they can be seeded at 0.5-1.0 x 104cells/cm2. Flasks will require passaging twice weekly at a 1:10 seeding density.
Media renewal: 2-3 times per week.
Culture Conditions: Incubate the culture at 37°C with 5% CO2.
Cryoprotectant medium: 10% DMSO + 90% FCS.
|Culture Medium:||EMEM + 20% Foetal Calf Serum (FCS) + 25mM Glucose + 2mM L-Glutamine|
|Depositor:||Dan Peet - University of Adelaide, South Australia|
|References:||Kittipassorn T, Haydinger CD, Wood JPM, Mammone T, Casson RJ, Peet DJ. Characterisation of the novel spontaneously immortalised rat Müller cell line SIRMu-1. Exp Eye Res. 2019 Apr; 181:127-135 PubMed: 30677389|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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