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UK Health Security Agency

EL4

EL4

Catalogue No.

85023105

Cell Line Name

EL4

Cell Line Description

Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen (J Nat Cancer Inst 1972;48:265) H-2b, IL-4 and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to PHA.

Characteristics

Products

A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2

Tissue of Origin

Blood

Karyotype

2n = 39

Applications

Tumourigenicity studies

Disease

Lymphoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

When starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100µl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and re-suspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium. Incubate flask at 37°C; 5 - 7% CO₂. Check daily. Subculture once exponential growth is established and maintain cultures between 3 - 9 x 100,000 cells/ml.

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof H Harris/Dr R Sutherland, Sir William Dunn School of Pathology, Oxford

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Br J Cancer 1950;4:372; Cancer Res 1965;25:813; J Immunol 1972;108:1146; J Nat Cancer Inst 1972;48:265

Bibliography

(1)J Biol Chem; 1996 May 31: 271(22): 13175-83"Cannabinoid inhibition of adenylate cyclase-mediated signal transduction and interleukin 2 (IL-2) expression in the murine T-cell line, EL4.IL-2."(2)Cell Signal; 1995 Jan: 7(1): 17-30"Rapid tyrosine phosphorylation of an 85,000 M(r) protein after phorbol ester stimulation of EL4 thymoma cells."(3)J Exp Med; 1994 Mar 1: 179(3): 1011-5"Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules."(4)Cancer Commun; 1990: 2(5): 181-8"The relationship between multidrug resistance and tumor necrosis factor resistance in an EL4 cell line model."(5)J Biol Chem; 1990 Feb 25: 265(6): 3146-52"Interleukin-1 signal transduction. Increased GTP binding and hydrolysis in membranes of a murine thymoma line (EL4)."(6)Int J Cancer; 1990 Mar 15: 45(3): 540-5"Cessation of autonomous proliferation of mouse lymphoma EL4 by fusion with a T cell line."(7)Eur J Immunol; 1989 Feb: 19(2): 329-34"Internalization of interleukin 1 (IL 1) correlates with IL 1-induced IL 2 receptor expression and IL 2 secretion of EL4 thymoma cells."(8)Eur J Immunol; 1987 May: 17(5): 605-11"Specific suppression elicited by EL4 lymphoma cells in syngeneic mice. Specificity includes self-antigens on EL4."(9)Transplant Proc; 1987 Feb: 19(1 Pt 1): 878-9"Characterization of the B6.C-H-2bm-1 (H-2bm-1) cytotoxic T lymphocyte clonal response to EL4."(10)Eur J Immunol; 1987 Jun: 17(6): 887-92"Limiting dilution assay for human B cells based on their activation by mutant EL4 thymoma cells: total and antimalaria responder B cell frequencies."(11)J Biol Chem; 1987 Jan 5: 262(1): 234-8"Phorbol esters induce interleukin 2 mRNA in sensitive but not in resistant EL4 cells."(12)J Immunol Methods; 1986 Apr 17: 88(2): 207-15"Sustained IL-2 production by the EL4 subline during continuous phorbol diester stimulation is related to an increase of IL-2-mRNA."(13)Biochem Biophys Res Commun; 1986 Oct 15: 140(1): 267-72"Lack of direct effect of 2,3,7,8-tetrachlorodibenzo-P-dioxin (TCDD) on protein kinase C activity in EL4 cells."(14)Cancer Res; 1986 Jun: 46(6): 3040-5"Substrates for protein kinase C in cytosol of EL4 mouse thymoma cells."(15)J Immunol; 1985 Dec: 135(6): 3944-50"Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. I. Functional characterization by monoclonal antibodies."(16)J Immunol; 1985 Dec: 135(6): 3951-7"Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. II. Structure, biosynthesis, and maturation."(17)Biochim Biophys Acta; 1985 Dec 19: 821(3): 479-87"Characterization of plasma membrane domains of mouse EL4 lymphoma cells obtained by affinity chromatography on concanavalin A-Sepharose."(18)J Immunol; 1984 Jul: 133(1): 7-9"The use of 5-azacytidine to establish constitutive interleukin 2-producing clones of the EL4 thymoma."(19)J Immunol; 1984 Jan: 132(1): 76-80"Cytotoxic T lymphocyte recognition of HLA-A/B antigens introduced into EL4 cells by cell-liposome fusion."(20)J Immunol; 1984 Jun: 132(6): 2767-74"Differential recognition and lysis of EL4 target cells by cytotoxic T cells: differences in H-2Kb antigenic density and cytoskeletal proteins."(21)J Immunol; 1984 May: 132(5): 2451-6"Partial purification and characterization of a BCGFII from EL4 culture supernatants."(22) Cell Immunol; 1984 Jan: 83(1): 107-23"Wheat germ agglutinin-resistant variant of EL4 containing altered oligosaccharides as a target cell for cytotoxic T cells."(23) Cancer Res; 1984 Oct: 44(10): 4263-6"Tumor and tissue distribution of a methotrexate-anti-EL4 immunoglobulin conjugate in EL4 lymphoma-bearing mice."(24)Proc Natl Acad Sci U S A; 1983 May: 80(9): 2642-6"Identification of high-affinity phorbol ester receptor in cytosol of EL4 thymoma cells: requirement for calcium, magnesium, and phospholipids."(25) Cancer Immunol Immunother; 1983: 14(3): 202-4"Synergistic anti-tumor effect of mini-cells prepared from Salmonella typhimurium with mitomycin C in EL4-bearing mice."(26)Biochim Biophys Acta; 1982 Jul 28: 689(2): 230-40"The relationship between plasma membrane lipid composition and physical-chemical properties. III. Detailed physical and biochemical analysis of fatty acid-substituted EL4 plasma membranes."(27)Cancer Res; 1982 May: 42(5): 1676-80"Identification of phorbol ester receptors in T-cell growth factor-producing and -nonproducing EL4 mouse thymoma cells."(28)J Biol Chem; 1982 Nov 25: 257(22): 13193-6"Decrease in cytosolic calcium/phospholipid-dependent protein kinase activity following phorbol ester treatment of EL4 thymoma cells."(29)Curr Top Microbiol Immunol; 1978: 81: 221-3"Attempts to produce cytolytically active cell hybrids using EL4 and MLC spleen."(30)J Histochem Cytochem; 1977 Jul: 25(7): 821-6"Flow system fluorescence polarization measurements on fluorescein diacetate-stained EL4 cells."(31)Eur J Cancer; 1977 Sep: 13(9): 925-35"Immunoprophylaxis and immunotherapy of EL4 lymphoma."(32)Int J Cancer; 1974 Sep 15: 14(3): 301-13"Detection of cytotoxic T lymphocytes in the EL4 mouse leukemia system: increased activity of immune spleen and peritoneal cells following preincubation and cell fractionation procedures."(33) Cell Immunol; 1974 May: 12(2): 280-9"Heterogenous populations of cytotoxic cells in the peritoneal cavity of BALB/c mice immunized with allogeneic EL4 leukemia cells."

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: EL4 (ECACC 85023105).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

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