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UK Health Security Agency

RAW 264

RAW 264

Catalogue No.

85062803

Cell Line Name

RAW 264

Cell Line Description

Established from an ascites of a tumour induced in a male mouse by intraperitoneal injection of Abelson Leukaemia virus (A-MuLV). Cells with pinocytose neutral red and phagocytose zymosan. Cells capable of antibody-dependent lysis of sheep erythrocytes and tumour targets. Growth inhibited by LPS (0.5ng/ml).

Characteristics

Receptors

Immunoglobulin and complement

Products

Lysozyme

Tissue of Origin

Blood

Karyotype

Not specified

Disease

Leukaemia

Culture Conditions

Cell Type

Macrophage

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²; 5% CO₂; 37°C. . Use cell scrapers to remove attached cells. Cells are semi-adherent, i.e. some cells grows in suspension, some loosely attach to the surface and others flattened out and attach to the flask. Cells should not be allowed to overgrow and become confluent as this can lead to loss of the flattened adherent cell characteristic.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Semi-adherent

Additional Info

Depositor

Dr W Scheirer, Sandoz Forschungsinstitut GmbH, Vienna

Country of Origin

Austria

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Immunol 1977;119:950; Cell 1978;15:261

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: RAW 264 (ECACC 85062803).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.