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UK Health Security Agency

MA104

MA104

Catalogue No.

85102918

Cell Line Name

MA104

Cell Line Description

Established from an African Green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11.Please note: The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators. Whitaker & Hayward (1985) found the cell line to originate from an African Green monkey and not a Rhesus macaque as was originally claimed. Whitaker AM & Hayward CJ (1985) The characterization of three monkey kidney cell lines. Develop. Biol. Standard. Vol 60. Pp 125 – 131. PMID: 4043530. Abstract: Three monkey kidney cell lines, Vero, GL-V3 and MA-104 were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. This finding was supported by data from isoenzyme studies.

General Info

Species

Monkey

Release Conditions

Restricted - CITES (Convention on the International Trade in Endangered Species of Wild Fauna and Flora) licence required for all orders outside the United Kingdom

Characteristics

Tissue of Origin

Kidney

Karyotype

Not specified

Applications

Virus studies: Simian rotavirus SA11

Disease

None Stated

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr A Collins, AFRC Institute for Research on Animal Diseases, Compton

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Gen Virol 1979;43:513; Arch Virol 1981;70:33; Whitaker AM & Hayward CJ (1985) The characterization of three monkey kidney cell lines.  Develop. Biol. Standard. Vol 60. Pp 125 - 131.  PMID: 4043530.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: MA104 (ECACC 85102918).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.