Culture Collections

General Cell Collection Detail

ECACC General Cell Collection: RPMI 1788

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 85112106
Cell Line Name: RPMI 1788
Other Collection No.: ATCC CCL 156
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: RPMI 1788 (ECACC 85112106)
Keywords: Human Caucasian peripheral blood lymphocyte
Cell Line Description:
Derived from the peripheral blood of an apparently normal 33-year-old Caucasian male. The cells secrete IgM (lambda light chain). HLA = A2, Aw33, B7, B14.

Cells express the gene for and produce immunoglobulin and Lymphotoxin (LT), also known as Tumour Necrosis Factor beta (TNFβ). EBV (EBNA) genome (also known as Human Herpes Virus 4) is present in very small quantities, based on the number of EBV genome equivalents/cell (detected by RNA-DNA hybridization). Therefore, cells are EBV/EBNA positive.
Species: Human
Tissue of Origin: blood
CellType: Lymphoblast
Growth Mode: Suspension
DNA Profile: STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19

Karyotype: 2n = 46, modal no. 45
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Maintain cultures between 3-4x100,000 cells/ml; 5% CO2; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.
Culture Medium: RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Depositor: Obtained from ATCC
Originator: No
Country: USA
Huang, C.C, Moore, G.E. Chromosomes of 14 hematopoietic cell lines derived from peripheral blood of persons with and without chromosome anomalies. J. Natl. Cancer Inst. 43: 1119-1128, 1969. PubMed: 53532

Minowada, J., Nonoyama, M., Moore, G.E., Rauch, A.M., and Pagano, J.S. The Presence of the Epstein-Barr Viral Genome in Human Lymphoblastoid B-Cell Lines and its absence in a Myeloma Cell Line. Cancer Res. 34: 1898-1903, 1974. Pubmed: 4366935

Kennedy, G., Kumano, J and Sugden B. Epstein-Barr Virus provides a Survival Factor to Burkitt’s Lymphomas. PNAS. 100 [24]: 14269-14274. 2003. Pubmed: 14603034

Aggarwal, B.B., Moffat, B and Harkins, R.N. Human Lymphotoxin. Production of a Lymphoblastoid Cell Line, Purification and Initial Characterization. J Biol. Chem. 259 [10]: 686-691. 1984. Pubmed: 6608523

Stone-Wolff, D. S., YIP, Y.K., Kelker, H.C., Le, J., Henriksen-Destefano, D., Rubin, B.Y., Rinderknecht, E., Aggarwal, B.B and Vilček, Interrelationships of human Interferon-Gamma with Lymphotoxin and Monocyte Cytotoxin. J. Exp Med 159: 828-843. 1984. Pubmed: 6421983
Additional Bibliography: Not specified
Patents: None specified by Depositor
Release Conditions: No

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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