|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||1321N1|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: 1321N1 (ECACC 86030402)|
|Keywords:||Human brain astrocytoma|
|Cell Line Description:||1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten (Pontén, J., Macintyre, E. H. (1968) PMID: 4313504).1321N1 has been shown to have very similar STR profile data to U-118 MG.|
|Tissue of Origin:||brain|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.|
|Culture Medium:||DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).|
|Depositor:||Dr M Shaw, ICI Pharmaceuticals Ltd|
|References:||Macintyre EH, Pontén J, Vatter AE. 1972 The ultrastructure of human and murine astrocytes and of human fibroblasts in culture. Acta Pathol Microbiol Scand A. 80(2):267-83 PMID: 4337530. Macintyre EH, Wintersgill CJ, Thormar H 1972 The establishment of a line of visna virus-producing human astrocytes (V-1181N1).Med Res Eng. 11(4):7-13 PMID: 4370460.|
|Additional Bibliography:||Foster SJ, Perkins JP. 1977 Glucocorticoids increase the responsiveness of cells in culture to prostaglandin E1.Proc Natl Acad Sci U S A. 74(11):4816-20 PMID: 200933. Barretina J, et al., 2012 The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity. Nature. 483(7391):603-7. PMID: 22460905.|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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