Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: CMT 93

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 89111413
Cell Line Name: CMT 93
Other Collection No.: ATCC CCL 223
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: CMT 93 (ECACC 89111413)
Keywords: Mouse rectum carcinoma
Cell Line Description: From a 19 month old male mouse (C57BL/ICRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.
Species: Mouse
Tissue of Origin: rectum
CellType: Epithelial
Growth Mode: Adherent
Karyotype: Hyperdiploid,modal no. 50
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Cells grow in clumps.
Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Depositor: Obtained from ATCC
Originator: No
Country: USA
References: J Pathol 1978;124:35
Additional Bibliography: Not specified
Patents: None specified by Depositor
Release Conditions: Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' form

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

Frozen
Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)
cDNA-20µl

Formats
  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

This product is only available through your local distributor.







Back to top
Copyright © Public Health England.

Please confirm your country of origin from the list below.