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Neuro 2a

Neuro 2a

Catalogue No.

89121404

Cell Line Name

Neuro 2a

Cell Line Description

Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.

Characteristics

Products

Microtubular protein

Tissue of Origin

Nerve

Morphology

Amoeboid-like

Karyotype

2n = 40

Applications

Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kinetics

Disease

Neuroblastoma

Culture Conditions

Cell Type

Neuronal

Subculture Routine

Split sub-confluent cultures (70-80%). The recommended seeding density for these cells is 2-4x104 cells/cm2. After resuscitation, these cells can take 3-4 days before a monolayer forms.

 

Recommended growth conditions 5% CO2; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

J Cell Biol 1969;43:69A; Proc Natl Acad Sci, USA 1970;65:129

Bibliography

Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: Neuro 2a (ECACC 89121404).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.