Culture Collections

General Cell Collection Detail





ECACC General Cell Collection: Neuro 2a

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 89121404
Cell Line Name: Neuro 2a
Other Collection No.: ATCC CCL 131
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: Neuro 2a (ECACC 89121404)
Keywords: Mouse Albino neuroblastoma
Cell Line Description: Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.
Species: Mouse
Tissue of Origin: nerve
CellType: Neuronal/amoeboid-like
Growth Mode: Adherent
Karyotype: 2n = 40
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4x10,000 cells cm²; 5% CO2; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.
Culture Medium: EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Depositor: Obtained from ATCC
Originator: No
Country: USA
References: J Cell Biol 1969;43:69A; Proc Natl Acad Sci, USA 1970;65:129
Additional Bibliography: Not specified
Patents: None specified by Depositor
Release Conditions: No

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Available Formats

Frozen
Growing
DNA-5µg (100ng/µl)
RNA-5µg (100ng/µl)
cDNA-20µl

Formats
  • Frozen
  • Growing
  • DNA-5µg (100ng/µl)
  • RNA-5µg (100ng/µl)
  • cDNA-20µl

This product is only available through your local distributor.







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