|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||HCT-8|
|Other Collection No.:||ATCC CCL 244|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: HCT-8 (ECACC 90032006)|
|Keywords:||Human ileocecal adenocarcinoma|
|Cell Line Description:||
From a 67 year old male.
The four cell lines: DLD-1 (90102540), HCT-15 (91030712), HCT-8 (90032006) and HRT-18 (86040306) have been shown to have a common genetic origin see Vermeulen SJ, Chen TR, Speleman F, Nollet F, Van Roy FM, Mareel MM 1998 Did the four human cancer cell lines DLD-1, HCT-15, HCT-8, and HRT-18 originate from one and the same patient? Cancer Genet Cytogenet. 107(1):76-9. PMID: 9809040. This has been confirmed at ECACC by STR profiling.>
|Tissue of Origin:||ileum|
|Karyotype:||2n = 46, pseudodiploid|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.|
|Culture Medium:||RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS) or 5% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS).|
|Depositor:||Obtained from ATCC|
|References:||Tompkins WA, Watrach AM, Schmale JD, Schultz RM, Harris JA 1974 Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J Natl Cancer Inst. 52(4):1101-10 PMID: 4826581.|
|Additional Bibliography:||Nelson-Rees WA, Flandermeyer RR, Hawthorne PK 1975 Distinctive banded marker chromosomes of human tumor cell lines. Int J Cancer. 16(1):74-82 PMID: 1058173.|
|Patents:||None specified by Depositor|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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