|Supplied by:||European Collection of Authenticated Cell Cultures (ECACC)|
|Culture Type:||Cell line|
|Collection:||ECACC General Collection|
|Cell Line Name:||MDA-MB-157|
|Citation Guidance:||If use of this culture results in a scientific publication, it should be cited in the publication as: MDA-MB-157 (ECACC 92020422)|
|Keywords:||Human breast medulla carcinoma|
|Cell Line Description:||MDA-MB-157 was established from the pleural effusion of a 44 year old woman with metastatic breast cancer. Desmosomes, microvilli and tonofilaments observed at boundaries between cells. Ethnicity: Black.|
|Tissue of Origin:||Breast|
|Karyotype:||Range 52-69; modal number 52-54|
Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.
ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
|Subculture Routine:||This cell line is relatively slow growing and should be left 5-7 days between splits (media change every 2-3 days). When resuscitating from frozen seed into non-vented flasks at 4-5 x10,000 cells/cm². Split sub-confluent cultures (80-90 %) 1:3 to 1:6, i.e. seeding at 1-3 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. CO2 is not required.|
|Culture Medium:||L15 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).|
|Depositor:||The University of Texas M. D. Anderson Cancer Center Office of Technology Commercialization 7515 S. Main, Suite 490, Unit 0510 Houston, Texas 77030|
|References:||Young RK, Cailleau RM, Mackay B, Reeves WJ 1974 Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro. 9(4):239-45. PMID: 4471183.|
|Additional Bibliography:||Cailleau R, Young R, Olivé M, Reeves WJ Jr 1974 Breast tumor cell lines from pleural effusions. J Natl Cancer Inst. 53(3):661-74. PMID: 4412247. Cruciger QV, Pathak S, Cailleau R.1976 Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet Cell Genet. 17(4):231-5. PMID: 1001030. Cailleau R, Olivé M, Cruciger QV 1978 Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro. 14(11):911-5.PMID: 730202. Brinkley BR, Beall PT, Wible LJ, Mace ML, Turner DS, Cailleau RM 1980 Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res.;40(9):3118-29.PMID: 7000337. Siciliano MJ, Barker PE, Cailleau R 1979 Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res.39(3):919-22. PMID: 427779. Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL 1994 Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54(10):2615-21. PMID: 8168088.|
|Patents:||None specified by Depositor|
|Release Conditions:||Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form.|
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
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