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HEL92.1.7

HEL92.1.7

Catalogue No.

92111706

Cell Line Name

HEL92.1.7

Cell Line Description

Derived from 30 year old male Caucasian. Expresses ß-2 microglobulin and Ia antigen. Used in the study of erythroid cell differentiation and globin gene expression. Analogous to Friend cell leukaemia in mice. K-562 has similar characteristics.

Characteristics

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10
D5S818: 11
D7S820: 7
D13S317: 9,11
D16S539: 11
TH01: 7
TPOX: 11
vWA: 14,17

Karyotype

Not specified

Applications

Erythroid cell differentiation studies, induced globin synthesis

Disease

Erythroleukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth can be very slow on resuscitation. Stand the culture flask up overnight using 20% FBS.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Science 1982;261:1233

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: HEL92.1.7 (ECACC 92111706).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.