Culture Collections

General Cell Collection Detail

ECACC General Cell Collection: Colo 794

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 94072237
Cell Line Name: Colo 794
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: Colo 794 (ECACC 94072237)
Keywords: Human melanoma
Cell Line Description: Colo 794 was established from a 14 year old male patient with a malignant melanoma. The melanoma showed rapid growth and metastases in the patient and the biopsy was taken before therapy from a subcutaneous metastasis. The cell line was eradicated from mycoplasma at ECACC.

COLO 794 and COLO 800 (ECACC Catalogue number 93051123) were both derived from the same 14 year old male This has been confirmed by STR profiling.

Species: Human
Tissue of Origin: Unknown
Growth Mode: Adherent
DNA Profile: STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 11
D5S818: 11
D7S820: 10,11
THO1: 6
TPOX: 11
vWA: 16,20

Karyotype: Not specified
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C; cells can show extensive blebs
Culture Medium: RPMI 1640+ 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Depositor: Dr. G. Moore, Denver General Hospital, Colorado Oncology Foundation, Denver, Colorado, USA
Originator: Yes
Country: USA
References: Unpublished
Additional Bibliography: Not specified
Patents: None specified by Depositor
Release Conditions: No

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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